FN Archimer Export Format
PT J
TI Optimizing sperm cryopreservation in great scallop (Pecten maximus)
BT 
AF SUQUET, Marc
   MALO, Florent
   GOURTAY, Clemence
   RATISKOL, Dominique
   RIOBE, Marion
   LE GRAND, Jacqueline
   QUEAU, Isabelle
   MINGANT, Christian
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;8:1;
FF 1:PDG-RBE-PFOM-PI;2:PDG-RBE-LEADNC;3:PDG-RBE-PFOM-ARN;4:PDG-RBE-PFOM-PI;5:PDG-RBE-PFOM-PI;6:PDG-RBE-PFOM-PI;7:PDG-RBE-PFOM-PI;8:PDG-RBE-PFOM-PI;
C1 IFREMER, Stn Expt Argenton, PFOM Dept, UMR 6539, 11 Presquile du Vivier, F-29840 Argenton, France.
C2 IFREMER, FRANCE
SI ARGENTON
   NOUMEA
   BREST
SE PDG-RBE-PFOM-PI
   PDG-RBE-LEADNC
   PDG-RBE-PFOM-ARN
UM LEMAR
IN WOS Ifremer jusqu'en 2018
IF 0.448
TC 1
UR https://archimer.ifremer.fr/doc/00359/47033/46946.pdf
LA English
DT Article
DE ;Pecten maximus;mollusc;sperm;cryopreservation;larval rearing
AB A basic cryopreservation protocol for great scallop sperm was recently published, with which thawed sperm survival remained limited to 25% of control values (Suquet et al. 2014). We designed here a set of five experiments to test ways of improving thawed sperm survival and storage by studying the effects of (1) cryoprotectant concentration; (2) sugar incorporation in the extender; (3) egg yolk addition to the extender; (4) sperm dilution rate; and (5) storage capacity of thawed sperm at 4 degrees C. A sixth experiment was then performed to compare rearing performances of larvae produced using thawed sperm from the optimal protocol defined in the present study with those produced using fresh sperm. During the first experiment, a significantly higher percentage of motile spermatozoa was recorded after thawing when the treatment had included 15 or 20% polyethylene glycol (PEG). In the second experiment, no significant improvement in post-thaw sperm motility was recorded when trehalose or glucose was added to the extender. In the third experiment, adding 10 or 20% egg yolk to the extender significantly decreased the percentage of motile sperm. During the fourth experiment, significantly higher percentages of motile sperm were observed for dilution rates ranging from 3 : 1 to 1 : 1 than for higher dilution rates. In the fifth experiment, a significant decrease in the percentage of post-thawed sperm was observed for storage durations of 30 and 60 min, relative to shorter durations. During the last experiment, similar rearing performances were observed between larvae produced using fresh and thawed sperm, up to 9 days post fertilization. In conclusion, the high survival of sperm recorded after thawing (67% of the control level) and the high rearing performances of progenies obtained using thawed sperm validate the use of the present improved cryopreservation protocol for sperm cryobanking in great scallop.
PY 2016
PD APR
SO Aquatic Living Resources
SN 0990-7440
PU Edp Sciences S A
VL 29
IS 2
UT 000386763900007
DI 10.1051/alr/2016017
ID 47033
ER

EF