Endemicity of the cosmopolitan mesophilic chemolithoautotroph Sulfurimonas at deep-sea hydrothermal vents

Type Article
Date 2017-04
Language English
Author(s) Mino Sayaka1, Nakagawa Satoshi2, 3, Makita Hiroko3, Toki Tomohiro4, Miyazaki Junichi3, Sievert Stefan M.5, Polz Martin F.6, Inagaki Fumio7, 8, Godfroy AnneORCID9, Kato Shingo10, Watanabe Hiromi11, Nunoura Takuro12, Nakamura Koichi13, Imachi Hiroyuki3, Watsuji Tomo-O3, Kojima Shigeaki14, Takai Ken3, Sawabe Tomoo1
Affiliation(s) 1 : Hokkaido Univ, Fac Fisheries Sci, Microbiol Lab, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611, Japan.
2 : Kyoto Univ, Grad Sch Agr, Div Appl Biosci, Lab Marine Environm Microbiol, Kyoto, Japan.
3 : Japan Agcy Marine Earth Sci & Technol JAMSTEC, Dept Subsurface Geobiol Anal & Res D SUGAR, Yokosuka, Kanagawa, Japan.
4 : Univ Ryukyus, Fac Sci, Dept Chem Biol & Marine Sci, Nishihara, Okinawa, Japan.
5 : Woods Hole Oceanog Inst, Dept Biol, Woods Hole, MA 02543 USA.
6 : MIT, Dept Civil & Environm Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA.
7 : JAMSTEC, Kochi Inst Core Sample Res, Nankoku, Kochi, Japan.
8 : JAMSTEC, Res & Dev Ctr Ocean Drilling Sci ODS, Yokohama, Kanagawa, Japan.
9 : IFREMER, UMR6197, Lab Microbiol Environm Extremes, Plouzane, France.
10 : RIKEN BioResource Ctr, Japan Collect Microorganisms, Tsukuba, Ibaraki, Japan.
11 : JAMSTEC, Dept Marine Biodivers Res, Yokosuka, Kanagawa, Japan.
12 : JAMSTEC, Res & Dev Ctr Marine Biosci, Yokosuka, Kanagawa, Japan.
13 : Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki, Japan.
14 : Univ Tokyo, Atmosphere & Ocean Res Inst, Kashiwa, Chiba, Japan.
Source Isme Journal (1751-7362) (Nature Publishing Group), 2017-04 , Vol. 11 , N. 4 , P. 909-919
DOI 10.1038/ismej.2016.178
WOS© Times Cited 15
Abstract Rich animal and microbial communities have been found at deep-sea hydrothermal vents. Although the biogeography of vent macrofauna is well understood, the corresponding knowledge about vent microbial biogeography is lacking. Here, we apply the multilocus sequence analysis (MLSA) to assess the genetic variation of 109 Sulfurimonas strains with greater than or equal to98% 16S rRNA gene sequence similarity, which were isolated from four different geographical regions (Okinawa Trough (OT), Mariana Volcanic Arc and Trough (MVAT), Central Indian Ridge (CIR) and Mid-Atlantic Ridge (MAR)). Sequence typing based on 11 protein-coding genes revealed high genetic variation, including some allele types that are widespread within regions, resulting in 102 nucleotide sequence types (STs). This genetic variation was predominantly due to mutation rather than recombination. Phylogenetic analysis of the 11 concatenated genes showed a clear geographical isolation corresponding to the hydrothermal regions they originated from, suggesting limited dispersal. Genetic differentiation among Sulfurimonas populations was primarily influenced by geographical distance rather than gas composition of vent fluid or habitat, although in situ environmental conditions of each microhabitat could not be examined. Nevertheless, Sulfurimonas may possess a higher dispersal capability compared with deep-sea hydrothermal vent thermophiles. This is the first report on MLSA of deep-sea hydrothermal vent Epsilonproteobacteria, which is indicative of allopatric speciation.
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Mino Sayaka, Nakagawa Satoshi, Makita Hiroko, Toki Tomohiro, Miyazaki Junichi, Sievert Stefan M., Polz Martin F., Inagaki Fumio, Godfroy Anne, Kato Shingo, Watanabe Hiromi, Nunoura Takuro, Nakamura Koichi, Imachi Hiroyuki, Watsuji Tomo-O, Kojima Shigeaki, Takai Ken, Sawabe Tomoo (2017). Endemicity of the cosmopolitan mesophilic chemolithoautotroph Sulfurimonas at deep-sea hydrothermal vents. Isme Journal, 11(4), 909-919. Publisher's official version : https://doi.org/10.1038/ismej.2016.178 , Open Access version : https://archimer.ifremer.fr/doc/00364/47539/