FN Archimer Export Format PT J TI Endemicity of the cosmopolitan mesophilic chemolithoautotroph Sulfurimonas at deep-sea hydrothermal vents BT AF MINO, Sayaka NAKAGAWA, Satoshi MAKITA, Hiroko TOKI, Tomohiro MIYAZAKI, Junichi SIEVERT, Stefan M. POLZ, Martin F. INAGAKI, Fumio GODFROY, Anne KATO, Shingo WATANABE, Hiromi NUNOURA, Takuro NAKAMURA, Koichi IMACHI, Hiroyuki WATSUJI, Tomo-o KOJIMA, Shigeaki TAKAI, Ken SAWABE, Tomoo AS 1:1;2:2,3;3:3;4:4;5:3;6:5;7:6;8:7,8;9:9;10:10;11:11;12:12;13:13;14:3;15:3;16:14;17:3;18:1; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:PDG-REM-EEP-LMEE;10:;11:;12:;13:;14:;15:;16:;17:;18:; C1 Hokkaido Univ, Fac Fisheries Sci, Microbiol Lab, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611, Japan. Kyoto Univ, Grad Sch Agr, Div Appl Biosci, Lab Marine Environm Microbiol, Kyoto, Japan. Japan Agcy Marine Earth Sci & Technol JAMSTEC, Dept Subsurface Geobiol Anal & Res D SUGAR, Yokosuka, Kanagawa, Japan. Univ Ryukyus, Fac Sci, Dept Chem Biol & Marine Sci, Nishihara, Okinawa, Japan. Woods Hole Oceanog Inst, Dept Biol, Woods Hole, MA 02543 USA. MIT, Dept Civil & Environm Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA. JAMSTEC, Kochi Inst Core Sample Res, Nankoku, Kochi, Japan. JAMSTEC, Res & Dev Ctr Ocean Drilling Sci ODS, Yokohama, Kanagawa, Japan. IFREMER, UMR6197, Lab Microbiol Environm Extremes, Plouzane, France. RIKEN BioResource Ctr, Japan Collect Microorganisms, Tsukuba, Ibaraki, Japan. JAMSTEC, Dept Marine Biodivers Res, Yokosuka, Kanagawa, Japan. JAMSTEC, Res & Dev Ctr Marine Biosci, Yokosuka, Kanagawa, Japan. Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki, Japan. Univ Tokyo, Atmosphere & Ocean Res Inst, Kashiwa, Chiba, Japan. C2 UNIV HOKKAIDO, JAPAN UNIV KYOTO, JAPAN JAMSTEC, JAPAN UNIV RYUKYUS, JAPAN WHOI, USA MIT, USA JAMSTEC, JAPAN JAMSTEC, JAPAN IFREMER, FRANCE RIKEN BRC, JAPAN JAMSTEC, JAPAN JAMSTEC, JAPAN AIST, JAPAN UNIV TOKYO, JAPAN SI BREST SE PDG-REM-EEP-LMEE UM BEEP-LM2E IN WOS Ifremer jusqu'en 2018 copubli-int-hors-europe IF 9.52 TC 18 UR https://archimer.ifremer.fr/doc/00364/47539/47557.pdf https://archimer.ifremer.fr/doc/00364/47539/47558.pdf LA English DT Article AB Rich animal and microbial communities have been found at deep-sea hydrothermal vents. Although the biogeography of vent macrofauna is well understood, the corresponding knowledge about vent microbial biogeography is lacking. Here, we apply the multilocus sequence analysis (MLSA) to assess the genetic variation of 109 Sulfurimonas strains with greater than or equal to98% 16S rRNA gene sequence similarity, which were isolated from four different geographical regions (Okinawa Trough (OT), Mariana Volcanic Arc and Trough (MVAT), Central Indian Ridge (CIR) and Mid-Atlantic Ridge (MAR)). Sequence typing based on 11 protein-coding genes revealed high genetic variation, including some allele types that are widespread within regions, resulting in 102 nucleotide sequence types (STs). This genetic variation was predominantly due to mutation rather than recombination. Phylogenetic analysis of the 11 concatenated genes showed a clear geographical isolation corresponding to the hydrothermal regions they originated from, suggesting limited dispersal. Genetic differentiation among Sulfurimonas populations was primarily influenced by geographical distance rather than gas composition of vent fluid or habitat, although in situ environmental conditions of each microhabitat could not be examined. Nevertheless, Sulfurimonas may possess a higher dispersal capability compared with deep-sea hydrothermal vent thermophiles. This is the first report on MLSA of deep-sea hydrothermal vent Epsilonproteobacteria, which is indicative of allopatric speciation. PY 2017 PD APR SO Isme Journal SN 1751-7362 PU Nature Publishing Group VL 11 IS 4 UT 000397281200009 BP 909 EP 919 DI 10.1038/ismej.2016.178 ID 47539 ER EF