FN Archimer Export Format
PT J
TI Irradiation effects on antibody performance in the frame of biochip-based instruments development for space exploration
BT 
AF BAQUE, M.
   DOBRIJEVIC, M.
   LE POSTOLLEC, A.
   MOREAU, T.
   FAYE, C.
   VIGIER, F.
   INCERTI, S.
   COUSSOT, G.
   CARON, J.
   VANDENABEELE TRAMBOUZE, Odile
AS 1:1;2:2,3;3:2,3;4:4;5:5;6:;7:6,7;8:8;9:9;10:10,11,12;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;
C1 Univ Roma Tor Vergata, Dept Biol, Rome, Italy.
   Univ Bordeaux, LAB, UMR 5804, F-33270 Florac, France.
   CNRS, LAB, UMR 5804, F-33270 Florac, France.
   Univ Clermont Ferrand, INSERM U1103, GReD, CNRS UMR6293, Aubiere, France.
   Cap Alpha, COLCOM, F-34830 Clapiers, France.
   Univ Bordeaux, CENBG, UMR 5797, F-33170 Gradignan, France.
   CNRS, IN2P3, CENBG, UMR 5797, F-33170 Gradignan, France.
   Univ Montpellier, Inst Biomol Max Mousseron, Fac Pharm, CNRS,Unite Mixte Rech 5247, F-34093 Montpellier 5, France.
   Inst Bergonie, Dept Radiotherapy, Ctr Comprehens Canc, F-33076 Bordeaux, France.
   Univ Bretagne Occidentale, LMEE, UEB, IUEM UMR 6197, Plouzane, France.
   CNRS, IUEM UMR 6197, LMEE, Plouzane, France.
   IFREMER, UMR6197, LMEE, Plouzane, France.
C2 UNIV ROMA TOR VERGATA, ITALY
   UNIV BORDEAUX, FRANCE
   CNRS, FRANCE
   UNIV CLERMONT FERRAND, FRANCE
   CAP ALPHA, FRANCE
   UNIV BORDEAUX, FRANCE
   CNRS, FRANCE
   UNIV MONTPELLIER, FRANCE
   INST BERGONIE, FRANCE
   UBO, FRANCE
   CNRS, FRANCE
   IFREMER, UMR6197, LMEE, Plouzane, France.
UM BEEP-LM2E
IF 1.921
TC 12
UR https://archimer.ifremer.fr/doc/00366/47678/47809.pdf
LA English
DT Article
DE ;astrobiology;biochip;cosmic rays;search for extraterrestrial life
AB Several instruments based on immunoassay techniques have been proposed for life-detection experiments in the framework of planetary exploration but few experiments have been conducted so far to test the resistance of antibodies against cosmic ray particles. We present several irradiation experiments carried out on both grafted and free antibodies for different types of incident particles (protons, neutrons, electrons and C-12) at different energies (between 9 MeV and 50 MeV) and different fluences. No loss of antibodies activity was detected for the whole set of experiments except when considering protons with energy between 20 and 30 MeV (on free and grafted antibodies) and fluences much greater than expected for a typical planetary mission to Mars for instance. Our results on grafted antibodies suggest that biochip-based instruments must be carefully designed according to the expected radiation environment for a given mission. In particular, a surface density of antibodies much larger than the expected proton fluence would prevent significant loss of antibodies activity and thus assuring a successful detection.
PY 2017
PD JAN
SO International Journal Of Astrobiology
SN 1473-5504
PU Cambridge Univ Press
VL 16
IS 1
UT 000390350600009
BP 82
EP 90
DI 10.1017/S1473550415000555
ID 47678
ER

EF