FN Archimer Export Format PT J TI Sunscreen, antioxidant, and bactericide capacities of phlorotannins from the brown macroalga Halidrys siliquosa BT AF LE LANN, Klervi SURGET, Gwladys COUTEAU, Celine COIFFARD, Laurence CERANTOLA, Stephane GAILLARD, Fanny LARNICOL, Maud ZUBIA, Mayalen GUERARD, Fabienne POUPART, Nathalie STIGER-POUVREAU, Valerie AS 1:1;2:1;3:2;4:2;5:3;6:4;7:5;8:6;9:1;10:1;11:1; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:; C1 UBO, European Inst Marine Studies IUEM, LEMAR UMR UBO CNRS Ifremer IRD 6539, Technopole Brest Iroise, F-29280 Plouzane, France. Nantes Atlant Univ, Univ Nantes, Fac Pharm, LPiC,MMS,EA2160, 9 Rue Bias,BP 53508, F-44000 Nantes, France. UBO, RMN RPE MS, 6 Ave,Victor Le Gorgeu CS93837, F-29238 Brest 3, France. CNRS, Plateforme Spectrometrie Masse MetaboMER, FR2424, Stn Biol, Pl Georges Teissier,BP 74, F-29682 Roscoff, France. Venelle Carros, Labs Sci & Mer, CS 70002, F-29480 Le Relecq Kerhuon, France. Univ Polynesie Francaise, EIO UMR 244, LabEx CORAIL, BP 6570, Faaa 98702, Tahiti, Fr Polynesia. C2 UBO, FRANCE UNIV NANTES, FRANCE UBO, FRANCE CNRS, FRANCE VENELLE CARROS, FRANCE UNIV POLYNESIE FRANCAISE, FRANCE UM LEMAR IF 2.616 TC 63 UR https://archimer.ifremer.fr/doc/00366/47682/47970.pdf LA English DT Article DE ;Antioxidant;Bactericidal;Phenolic compounds;Phaeophyceae;Seaweed;ORAC value;Sunscreen potential AB The present study focused on a brown macroalga (Halidrys siliquosa), with a particular emphasis on polyphenols and their associated biological activities. Two fractions were obtained by liquid/liquid purification from a crude hydroethanolic extract: (i) an ethyl acetate fraction and (ii) an aqueous fraction. Total phenolic contents and antioxidant activities of extract and both fractions were assessed by in vitro tests (Folin-Ciocalteu test, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power assay, superoxide anion scavenging assay, and beta-carotene-linoleic acid system). For the most active fraction, i.e., the ethyl acetate fraction, the oxygen radical absorbance capacity (ORAC) value, antibacterial activities, and sunscreen potential (Sun Protection Factor and UV-A-Protection Factor) were tested in vitro. A high correlation found between antioxidant activities and total phenolic content was interpreted as the involvement of polyphenolic compounds in antioxidant mechanisms. Interestingly, the ethyl acetate fraction appeared to be a broad-spectrum UV absorber and showed a strong bactericidal activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. In this fraction, four phenolic compounds (trifuhalols and tetrafuhalols and, for the first time, diphlorethols and triphlorethols) were identified using 1D and 2D nuclear magnetic resonance (NMR) and MS analysis. These findings are promising for the use of H. siliquosa, abundant in Brittany, as a valuable source of photoprotectant molecules for sunscreen and cosmetic applications. PY 2016 PD DEC SO Journal Of Applied Phycology SN 0921-8971 PU Springer VL 28 IS 6 UT 000390090300038 BP 3547 EP 3559 DI 10.1007/s10811-016-0853-0 ID 47682 ER EF