Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas

Type Article
Date 2017-01
Language English
Author(s) Boullot Floriane1, Castrec Justine1, Bidault Adeline1, Dantas Natanael2, Payton Laura3, Perrigault Mickael3, Tran Damien3, Amzil Zouher4, Boudry PierreORCID5, Soudant Philippe1, Hegaret HeleneORCID1, Fabioux Caroline1
Affiliation(s) 1 : Univ Bretagne Occidentale, Lab Sci Environm Marin LEMAR, Inst Univ Europeen Mer, UMR 6539,CNRS,UBO,IRD,Ifremer, F-29280 Plouzane, France.
2 : Univ Fed Paraiba, Dept Mol Biol, Exact & Nat Sci Ctr, Lab Immunol & Pathol Invertebrates, Campus 1, BR-58051900 Joao Pessoa, Paraiba, Brazil.
3 : Univ Bordeaux, Stn Marine Arcachon, Equipe Ecotoxicol Aquat, UMR 5805,EPOC,CNRS, F-33120 Arcachon, France.
4 : IFREMER, Lab Phycotoxines, BP 21105, F-44311 Nantes, France.
5 : IFREMER, UMR 6539, LEMAR, CNRS,UBO,IRD, F-29280 Plouzane, France.
Source Marine Drugs (1660-3397) (Mdpi Ag), 2017-01 , Vol. 15 , N. 1 , P. 21 (1-23)
DOI 10.3390/md15010021
WOS© Times Cited 5
Keyword(s) Crassostrea gigas, sodium channel, alternative splicing, Alexandrium minutum, paralytic shellfish toxins
Abstract Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized. The expression of CgNav1, analyzed by in situ hybridization, is specific to nervous cells and to structures corresponding to neuromuscular junctions. Real-time PCR analyses showed a strong expression of CgNav1A in the striated muscle while CgNav1B is mainly expressed in visceral ganglia. CgNav1C expression is ubiquitous. The PST binding site (domain II) of CgNav1 variants possess an amino acid Q that could potentially confer a partial saxitoxin (STX)-resistance to the channel. The CgNav1 genotype or alternative splicing would not be the key point determining PST bioaccumulation level in oysters.
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Boullot Floriane, Castrec Justine, Bidault Adeline, Dantas Natanael, Payton Laura, Perrigault Mickael, Tran Damien, Amzil Zouher, Boudry Pierre, Soudant Philippe, Hegaret Helene, Fabioux Caroline (2017). Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas. Marine Drugs, 15(1), 21 (1-23). Publisher's official version : https://doi.org/10.3390/md15010021 , Open Access version : https://archimer.ifremer.fr/doc/00368/47877/