FN Archimer Export Format
PT J
TI Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas
BT 
AF BOULLOT, Floriane
   CASTREC, Justine
   BIDAULT, Adeline
   DANTAS, Natanael
   PAYTON, Laura
   PERRIGAULT, Mickael
   TRAN, Damien
   AMZIL, Zouher
   BOUDRY, Pierre
   SOUDANT, Philippe
   HEGARET, Helene
   FABIOUX, Caroline
AS 1:1;2:1;3:1;4:2;5:3;6:3;7:3;8:4;9:5;10:1;11:1;12:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:PDG-ODE-DYNECO-PHYC;9:PDG-RBE-PFOM;10:;11:;12:;
C1 Univ Bretagne Occidentale, Lab Sci Environm Marin LEMAR, Inst Univ Europeen Mer, UMR 6539,CNRS,UBO,IRD,Ifremer, F-29280 Plouzane, France.
   Univ Fed Paraiba, Dept Mol Biol, Exact & Nat Sci Ctr, Lab Immunol & Pathol Invertebrates, Campus 1, BR-58051900 Joao Pessoa, Paraiba, Brazil.
   Univ Bordeaux, Stn Marine Arcachon, Equipe Ecotoxicol Aquat, UMR 5805,EPOC,CNRS, F-33120 Arcachon, France.
   IFREMER, Lab Phycotoxines, BP 21105, F-44311 Nantes, France.
   IFREMER, UMR 6539, LEMAR, CNRS,UBO,IRD, F-29280 Plouzane, France.
C2 UBO, FRANCE
   UNIV FED PARAIBA, BRAZIL
   UNIV BORDEAUX, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI NANTES
   BREST
SE PDG-ODE-DYNECO-PHYC
   PDG-RBE-PFOM
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 4.379
TC 13
UR https://archimer.ifremer.fr/doc/00368/47877/47892.pdf
LA English
DT Article
DE ;Crassostrea gigas;sodium channel;alternative splicing;Alexandrium minutum;paralytic shellfish toxins
AB Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized. The expression of CgNav1, analyzed by in situ hybridization, is specific to nervous cells and to structures corresponding to neuromuscular junctions. Real-time PCR analyses showed a strong expression of CgNav1A in the striated muscle while CgNav1B is mainly expressed in visceral ganglia. CgNav1C expression is ubiquitous. The PST binding site (domain II) of CgNav1 variants possess an amino acid Q that could potentially confer a partial saxitoxin (STX)-resistance to the channel. The CgNav1 genotype or alternative splicing would not be the key point determining PST bioaccumulation level in oysters.
PY 2017
PD JAN
SO Marine Drugs
SN 1660-3397
PU Mdpi Ag
VL 15
IS 1
UT 000392979600020
DI 10.3390/md15010021
ID 47877
ER

EF