FN Archimer Export Format
PT J
TI A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola
BT 
AF Pigné, Sandrine
   Zykwinska, Agata
   Janod, Etienne
   Cuenot, Stéphane
   Kerkoud, Mohammed
   Raulo, Roxane
   Bataillé-Simoneau, Nelly
   Marchi, Muriel
   Kwasiborski, Anthony
   N’Guyen, Guillaume
   Mabilleau, Guillaume
   Simoneau, Philippe
   Guillemette, Thomas
AS 1:1;2:2,3;3:2;4:2;5:1;6:1;7:1;8:1;9:1;10:1;11:4;12:1;13:1;
FF 1:;2:PDG-RBE-BRM-LEMMMB;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;
C1 IRHS, Agrocampus.Ouest, INRA, Universite d'Angers, SFR 4207 QuaSaV, 49071 Beaucouze, France
   UMR 6502, Institut des Materiaux Jean Rouxel, 2, Rue de la Houssiniere, BP 32229, 44322 Nantes Cedex 3, France
   Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, IFREMER, Rue de l’île d’Yeu, BP 21105, 44311 Nantes Cedex 3, France
   Plateforme SCIAM, Institut de Biologie en Santé, CHU, Université d’Angers, 4, Rue Larrey, 49933 Angers Cedex, France.
C2 INRA, FRANCE
   IMN, FRANCE
   IFREMER, FRANCE
   UNIV ANGERS, FRANCE
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN DOAJ
TC 0
UR https://archimer.ifremer.fr/doc/00371/48189/48305.pdf
   https://archimer.ifremer.fr/doc/00371/48189/48307.tiff
   https://archimer.ifremer.fr/doc/00371/48189/48308.png
   https://archimer.ifremer.fr/doc/00371/48189/48309.tiff
   https://archimer.ifremer.fr/doc/00371/48189/48310.pdf
LA English
DT Article
DE ;Monooxygenase;Cell wall;Melanin;Fungus;Flavoprotein
AB Background: Flavin-dependent monooxygenases are involved in key biological processes as they catalyze a wide variety of chemo-, regio- and enantioselective oxygenation reactions. Flavoprotein monooxygenases are frequently encountered in micro-organisms, most of which require further functional and biocatalytic assessment. Here we investigated the function of the AbMak1 gene, which encodes a group A flavin monooxygenase in the plant pathogenic fungus Alternaria brassicicola, by generating a deficient mutant and examining its phenotype. Results: Functional analysis indicates that the AbMak1 protein is involved in cell wall biogenesis and influences the melanization process. We documented a significant decrease in melanin content in the Äabmak1 strain compared to the wild-type and complemented strains. We investigated the cell wall morphology and physical properties in the wild-type and transformants using electron and atomic force microscopy. These approaches confirmed the aberrant morphology of the conidial wall structure in the Äabmak1 strain which had an impact on hydrophilic adhesion and conidial surface stiffness. However, there was no significant impairment in growth, conidia formation, pathogenicity or susceptibility to various environmental stresses in the Äabmak1 strain.  Conclusion: This study sheds new light on the function of a fungal flavin-dependent monooxygenase, which plays an important role in melanization.
PY 2017
PD DEC
SO Fungal Biology and Biotechnology
SN 2054-3085
PU Springer Nature
VL 4
IS 1
BP 1
EP 13
DI 10.1186/s40694-016-0029-3
ID 48189
ER

EF