FN Archimer Export Format
PT J
TI Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna (     Thunnus obesus     ) and Yellowfin Tuna (     Thunnus albacares     ), in Canned Tuna
BT 
AF BOJOLLY, Daline
   DOYEN, Perine
   LE FUR, Bruno
   CHRISTAKI, Urania
   VERREZ-BAGNIS, Veronique
   GRARD, Thierry
AS 1:1,6,8;2:1,2,3,5;3:8;4:6;5:7;6:1,2,3,4,5;
FF 1:;2:;3:;4:;5:PDG-RBE-BRM-LEMMMB;6:;
C1 Univ Littoral Cote dOpale, EA 7394, ICV, USC Anses ULCO, F-62200 Boulogne Sur Mer, France.
   Univ Lille, F-59000 Lille, France.
   Univ Artois, F-62000 Arras, France.
   INRA, Paris, France.
   ISA, F-59000 Lille, France.
   Lille 1, ULCO, CNRS, Lab Oceanol & Geosci,UMR 8187, F-62930 Wimereux, France.
   IFREMER, F-44300 Nantes, France.
   PFINV, F-62200 Boulogne Sur Mer, France.
C2 UNIV LITTORAL COTE D'OPALE, FRANCE
   UNIV LILLE, FRANCE
   UNIV ARTOIS, FRANCE
   INRA, FRANCE
   ISA, FRANCE
   UNIV LITTORAL COTE D'OPALE, FRANCE
   IFREMER, FRANCE
   PFINV, FRANCE
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 3.412
TC 25
UR https://archimer.ifremer.fr/doc/00371/48217/48403.pdf
LA English
DT Article
DE ;tuna;authentication;quantification;canned products;TaqMan;qPCR;species identification;bigeye tuna (Thunnus obesus);yellowfin tuna (Thunnus albacares)
AB Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one was based on absolute quantification using standard curves realized with these two markers; the second one was founded on relative quantification with the universal 12S rRNA gene as the endogenous gene. On the basis of our results, we conclude that our methodology could be applied to authenticate these two closely related tuna species when used in a binary mix in tuna cans.
PY 2017
PD FEB
SO Journal Of Agricultural And Food Chemistry
SN 0021-8561
PU Amer Chemical Soc
VL 65
IS 4
UT 000393355200028
BP 913
EP 920
DI 10.1021/acs.jafc.6b04713
ID 48217
ER

EF