FN Archimer Export Format
PT J
TI Transcriptomic Profiling of Egg Quality in Sea Bass (Dicentrarchus labrax) Sheds Light on Genes Involved in Ubiquitination and Translation
BT 
AF ZARSKI, Daniel
   LE CAM, Aurelie
   MONTFORT, Jerome
   DUTTO, Gilbert
   VIDAL, Marie-Odile
   FAUVEL, Christian
   BOBE, Julien
AS 1:1,2;2:3;3:3;4:3;5:4;6:4;7:4;8:3;
FF 1:;2:;3:;4:;5:PDG-RBE-MARBEC-LSEA;6:PDG-DOP-DCM-PM-APOI;7:PDG-RBE-MARBEC-L3AS;8:;
C1 Univ Warmia & Mazury, Dept Lake & River Fisheries, Ul Oczapowskiego 2, PL-10719 Olsztyn, Poland.
   Szent Istvan Univ, Dept Aquaculture, Pater Karoly 1, H-2100 Godollo, Hungary.
   INRA, LPGP, F-35000 Rennes, France.
   Univ Montpellier, Stn Expt Aquaculture, IFREMER, UMR MARBEC,IRD,CNRS, Chemin Maguelone, Palavas Les Flots 34250, France.
C2 UNIV WARMIA & MAZURY, POLAND
   UNIV SZENT ISTVAN, HUNGARY
   INRA, FRANCE
   IFREMER, FRANCE
SI PALAVAS
SE PDG-RBE-MARBEC-LSEA
   PDG-DOP-DCM-PM-APOI
   PDG-RBE-MARBEC-L3AS
UM MARBEC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-europe
IF 2.328
TC 25
UR https://archimer.ifremer.fr/doc/00371/48220/48345.pdf
LA English
DT Article
DE ;Microarray;Genomics;Transcriptomics;Aquaculture;Controlled reproduction
AB Variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. This stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. Molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. However, very few studies have been devoted to this aspect so far. Within this study, the microarray-based transcriptomic analysis of eggs (of different quality) of sea bass (Dicentrarchus labrax) was performed. An Agilent oligo microarray experiment was performed on labelled mRNA extracted from 16 batches of eggs (each batch obtained from a different female) of sea bass, in which over 24,000 published probe arrays were used. We identified 39 differentially expressed genes exhibiting a differential expression between the groups of low (fertilization rate < 60 %) and high (fertilization rate > 60 %) quality. The mRNA levels of eight genes were further analyzed by quantitative PCR. Seven genes were confirmed by qPCR to be differentially expressed in eggs of low and high quality. This study confirmed the importance of some of the genes already reported to be potential molecular quality indicators (mainly rnf213 and irf7), but we also found new genes (mainly usp5, mem-prot, plec, cenpf), which had not yet been reported to be quality-dependent in fish. These results suggest the importance of genes involved in several important processes, such as protein ubiquitination, translation, DNA repair, and cell structure and architecture; these probably being the mechanisms that contribute to egg developmental competence in sea bass.
PY 2017
PD FEB
SO Marine Biotechnology
SN 1436-2228
PU Springer
VL 19
IS 1
UT 000395086900009
BP 102
EP 115
DI 10.1007/s10126-017-9732-1
ID 48220
ER

EF