Use of low density polyethylene membranes for assessment of genotoxicity of PAHs in the Seine River
|Author(s)||Vincent-Hubert Francoise1, 2, Uher Emmanuelle1, Di Giorgio Carole3, Michel Cecile1, de Meo Michel3, Gourlay-France Catherine1|
|Affiliation(s)||1 : IRSTEA, UR Hydrosyst & Bioproc, 1 Rue Pierre Gilles de Gennes,CS10030, F-92761 Antony, France.
2 : IFREMER, Lab Microbiol LNR, Rue Ile Yeu,BP 21105, F-44311 Nantes 03, France.
3 : Univ Mediterranee, Biogenotoxicol & Mutagenese Environm EA 1784, Marseille, France.
|Source||Ecotoxicology (0963-9292) (Springer), 2017-03 , Vol. 26 , N. 2 , P. 165-172|
|WOS© Times Cited||4|
|Keyword(s)||Genotoxicity, River water, Passive samplers, PAHs, Ames assay, Umu test|
|Abstract||The genotoxicity of river water dissolved contaminants is usually estimated after grab sampling of river water. Water contamination can now be obtained with passive samplers that allow a time-integrated sampling of contaminants. Since it was verified that low density polyethylene membranes (LDPE) accumulate labile hydrophobic compounds, their use was proposed as a passive sampler. This study was designed to test the applicability of passive sampling for combined chemical and genotoxicity measurements. The LDPE extracts were tested with the umu test (TA1535/pSK1002 ± S9) and the Ames assay (TA98, TA100 and YG1041 ± S9). We describe here this new protocol and its application in two field studies on four sites of the Seine River. Field LDPE extracts were negative with the YG1041 and TA100 and weakly positive with the TA98 + S9 and Umu test. Concentrations of labile mutagenic PAHs were higher upstream of Paris than downstream of Paris. Improvement of the method is needed to determine the genotoxicity of low concentrations of labile dissolved organic contaminants.|