Genetic parameters of resistance to Vibrio aestuarianus, and OsHV-1 infections in the Pacific oyster, Crassostrea gigas, at three different life stages

Type Article
Date 2017-02
Language English
Author(s) Azema Patrick1, Lamy Jean-Baptiste1, Boudry PierreORCID2, Renault Tristan3, Travers Marie-AgnesORCID1, Degremont LionelORCID1
Affiliation(s) 1 : IFREMER, Lab Genet & Pathol Mollusques Marins, Ave Mus de Loup, F-17390 La Tremblade, France.
2 : IFREMER, Ctr Bretagne, Lab Sci Environm Marin, UBO,CNRS,IRD,UMR LEMAR 6539, CS 10070, F-29280 Plouzane, France.
3 : IFREMER, Dept Ressources Biol & Environm, Rue Ile dYeu, F-44300 Nantes, France.
Source Genetics Selection Evolution (0999-193X) (Biomed Central Ltd), 2017-02 , Vol. 49 , N. 23 , P. 1-16
DOI 10.1186/s12711-017-0297-2
WOS© Times Cited 10
Abstract Background

In France, two main diseases threaten Pacific oyster production. Since 2008, Crassostrea gigas spat have suffered massive losses due to the ostreid herpesvirus OsHV-1, and since 2012, significant mortalities in commercial-size adults have been related to infection by the bacterium Vibrio aestuarianus. The genetic basis for resistance to V. aestuarianus and OsHV-1 and the nature of the genetic correlation between these two traits were investigated by using 20 half-sib sire families, each containing two full-sib families. For each disease, controlled infectious challenges were conducted using naïve oysters that were 3 to 26 months old. In addition, siblings were tested under field, pond and raceway conditions to determine whether laboratory trials reflected mortality events that occur in the oyster industry.
Results

First, we estimated the genetic basis of resistance to V. aestuarianus in C. gigas. Susceptibility to the infection was low for oysters in spat stage but increased with later life stages. Second, we confirmed a strong genetic basis of resistance to OsHV-1 infection at early stages and demonstrated that it was also strong at later stages. Most families had increased resistance to OsHV-1 infection from the spat to adult stages, while others consistently showed low or high mortality rates related to OsHV-1 infection, regardless of the life stage. Our third main finding was the absence of genetic correlations between resistance to OsHV-1 infection and resistance to V. aestuarianus infection.
Conclusions

Selective breeding to enhance resistance to OsHV-1 infection could be achieved through selective breeding at early stages and would not affect resistance to V. aestuarianus infection. However, our results suggest that the potential to select for improved resistance to V. aestuarianus is lower. Selection for dual resistance to OsHV-1 and V. aestuarianus infection in C. gigas might reduce the impact of these two major diseases by selecting families that have the highest breeding values for resistance to both diseases.
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Additional file 1: Figure S1. Correlations between SAS and ASReml heritability estimates for survival in C. gigas when exposed to OsHV-1 or V. aestuarianus under controlled laboratory conditions ... 1 245 KB Open access
Additional file 2: Table S1. Variance components and narrow sense heritability for survival in C. gigas for each V. aestuarianus challenge under laboratory conditions (±SE). The data provided ... 1 247 KB Open access
Additional file 3: Table S2. Variance components and narrow sense heritability for survival in C. gigas for each OsHV-1 challenge under laboratory ... 1 246 KB Open access
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How to cite 

Azema Patrick, Lamy Jean-Baptiste, Boudry Pierre, Renault Tristan, Travers Marie-Agnes, Degremont Lionel (2017). Genetic parameters of resistance to Vibrio aestuarianus, and OsHV-1 infections in the Pacific oyster, Crassostrea gigas, at three different life stages. Genetics Selection Evolution, 49(23), 1-16. Publisher's official version : https://doi.org/10.1186/s12711-017-0297-2 , Open Access version : https://archimer.ifremer.fr/doc/00372/48350/