FN Archimer Export Format
PT J
TI Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups
BT 
AF SERIVE, Benoit
   NICOLAU, Elodie
   BERARD, Jean-Baptiste
   KAAS, Raymond
   PASQUET, Virginie
   PICOT, Laurent
   CADORET, Jean-Paul
AS 1:1;2:1;3:1;4:1;5:2;6:2;7:1;
FF 1:PDG-RBE-BRM-PBA;2:PDG-RBE-BRM-PBA;3:PDG-RBE-BRM-PBA;4:PDG-RBE-BRM-PBA;5:;6:;7:PDG-RBE-BRM;
C1 IFREMER, Lab Physiol & Biotechnol Algues, Nantes, France.
   Univ la Rochelle, UMRi CNRS LIENSs 7266, La Rochelle, France.
C2 IFREMER, FRANCE
   UNIV LA ROCHELLE, FRANCE
SI NANTES
SE PDG-RBE-BRM-PBA
   PDG-RBE-BRM
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-univ-france
IF 2.766
TC 34
UR https://archimer.ifremer.fr/doc/00373/48416/48627.pdf
   https://archimer.ifremer.fr/doc/00373/48416/48628.pdf
   https://archimer.ifremer.fr/doc/00373/48416/48629.pdf
   https://archimer.ifremer.fr/doc/00373/48416/48630.pdf
   https://archimer.ifremer.fr/doc/00373/48416/48631.pdf
   https://archimer.ifremer.fr/doc/00373/48416/48632.gephi
LA English
DT Article
AB Phytoplankton, with an estimated 30 000 to 1 000 000 species clustered in 12 phyla, presents a high taxonomic and ecophysiological diversity, reflected by the complex distribution of pigments among the different algal classes. High performance liquid chromatography is the gold standard method for qualitative and quantitative analysis of phytoplankton pigments in seawater and culture samples, but only a few pigments can be used as robust chemotaxonomic markers. A major challenge is thus to identify new ones, characteristic of a strain, species, class or taxon that cannot be currently identified on the basis of its pigment signature. Using an optimized extraction process coupled to a HPLC de-replication strategy, we examined the pigment composition of 37 microalgae strains, representative of the broad taxonomic diversity of marine and freshwater species (excluding cyanobacteria). For each species, the major pigments already described were unambiguously identified. We also observed the presence of several minor unidentified pigments in each chromatogram. The global analysis of pigment compositions revealed a total of 124 pigments, including 98 pigments or derivatives unidentified using the standards. Absorption spectra indicated that 35 corresponded to chlorophyll/porphyrin derivatives, 57 to carotenoids and six to derivatives having both spectral signatures. Sixty-one of these unidentified or new carotenoids and porphyrin derivatives were characteristic of particular strains or species, indicating their possible use as highly specific chemotaxonomic markers capable of identifying one strain out of the 37 selected. We developed a graphical analysis using Gephi software to give a clear representation of pigment communities among the various phytoplankton strains, and to reveal strain-characteristic and shared pigments. This made it possible to reconstruct the taxonomic evolution of microalgae classes, on the basis of the conservation, loss, and/or appearance of pigments.
PY 2017
PD FEB
SO Plos One
SN 1932-6203
PU Public Library Science
VL 12
IS 2
UT 000394682400023
DI 10.1371/journal.pone.0171872
ID 48416
ER

EF