Transcriptomic features of Pecten maximus oocyte quality and maturation

Type Article
Date 2017-03
Language English
Author(s) Pauletto Marianna1, Milan Massimo1, Huvet ArnaudORCID2, Corporeau Charlotte2, Suquet Marc2, Planas Josep V.3, 4, Moreira Rebeca5, Figueras Antonio5, Novoa Beatriz5, Patarnello Tomaso1, Bargelloni Luca1
Affiliation(s) 1 : Univ Padua, Dept Comparat Biomed & Food Sci, Padua, Italy.
2 : IFREMER, CNRS, UMR 6539,UBO,IRD, Lab Sci Environm Marin LEMAR, Plouzane, France.
3 : Univ Barcelona, Fac Biol, Dept Fisiol & Immunol, Barcelona, Spain.
4 : Univ Barcelona, Inst Biomed, Barcelona, Spain.
5 : Inst Invest Marinas IIM CSIC, Vigo, Pontevedra, Spain.
Source Plos One (1932-6203) (Public Library Science), 2017-03 , Vol. 12 , N. 3 , P. e0172805 (1-22)
DOI 10.1371/journal.pone.0172805
WOS© Times Cited 7
Abstract The king scallop Pecten maximus is a high valuable species of great interest in Europe for both fishery and aquaculture. Notably, there has been an increased investment to produce seed for enhancement programmes of wild scallop populations. However, hatchery production is a relatively new industry and it is still underdeveloped. Major hurdles are spawning control and gamete quality. In the present study, a total of 14 scallops were sampled in the bay of Brest (Brittany, France) to compare transcriptomic profiles of mature oocytes collected by spawning induction or by stripping. To reach such a goal, a microarray analysis was performed by using a custom 8x60K oligonucleotide microarray representing 45,488 unique scallop contigs. First we identified genes that were differentially expressed depending on oocyte quality, estimated as the potential to produce D-larvae. Secondly, we investigated the transcriptional features of both stripped and spawned oocytes. Genes coding for proteins involved in cytoskeletal dynamics, serine/threonine kinases signalling pathway, mRNA processing, response to DNA damage, apoptosis and cell-cycle appeared to be of crucial importance for both oocyte maturation and developmental competence. This study allowed us to dramatically increase the knowledge about transcriptional features of oocyte quality and maturation, as well as to propose for the first time putative molecular markers to solve a major bottleneck in scallop aquaculture.
Full Text
File Pages Size Access
Publisher's official version 22 1 MB Open access
S1 File. Pecten maximus transcriptome assembly 4 174 KB Open access
S2 File. Sequence of the contigs employed for the DNA microarray platform. 44 MB Open access
S1 Table. Blastx best hit of each probe represented in the microarray platform. 12 MB Open access
S2 Table. Correlation between gene expression and D-larval rate. 414 KB Open access
S3 Table. Enrichment analysis of transcripts correlated with D-larval rate. 18 KB Open access
S4 Table. DEGs between stripped and spawned oocytes. 96 KB Open access
S5 Table. Enrichment analysis of DEGs between stripped and spawned oocytes. 6 KB Open access
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Pauletto Marianna, Milan Massimo, Huvet Arnaud, Corporeau Charlotte, Suquet Marc, Planas Josep V., Moreira Rebeca, Figueras Antonio, Novoa Beatriz, Patarnello Tomaso, Bargelloni Luca (2017). Transcriptomic features of Pecten maximus oocyte quality and maturation. Plos One, 12(3), e0172805 (1-22). Publisher's official version : https://doi.org/10.1371/journal.pone.0172805 , Open Access version : https://archimer.ifremer.fr/doc/00374/48484/