FN Archimer Export Format PT J TI Transcriptomic features of Pecten maximus oocyte quality and maturation BT AF PAULETTO, Marianna MILAN, Massimo HUVET, Arnaud CORPOREAU, Charlotte SUQUET, Marc PLANAS, Josep V. MOREIRA, Rebeca FIGUERAS, Antonio NOVOA, Beatriz PATARNELLO, Tomaso BARGELLONI, Luca AS 1:1;2:1;3:2;4:2;5:2;6:3,4;7:5;8:5;9:5;10:1;11:1; FF 1:;2:;3:PDG-RBE-PFOM-PI;4:PDG-RBE-PFOM-PI;5:PDG-RBE-PFOM-PI;6:;7:;8:;9:;10:;11:; C1 Univ Padua, Dept Comparat Biomed & Food Sci, Padua, Italy. IFREMER, CNRS, UMR 6539,UBO,IRD, Lab Sci Environm Marin LEMAR, Plouzane, France. Univ Barcelona, Fac Biol, Dept Fisiol & Immunol, Barcelona, Spain. Univ Barcelona, Inst Biomed, Barcelona, Spain. Inst Invest Marinas IIM CSIC, Vigo, Pontevedra, Spain. C2 UNIV PADUA, ITALY IFREMER, FRANCE UNIV BARCELONA, SPAIN UNIV BARCELONA, SPAIN CSIC, SPAIN SI BREST ARGENTON SE PDG-RBE-PFOM-PI UM LEMAR IN WOS Ifremer jusqu'en 2018 DOAJ copubli-europe IF 2.766 TC 9 UR https://archimer.ifremer.fr/doc/00374/48484/48751.pdf https://archimer.ifremer.fr/doc/00374/48484/48752.pdf https://archimer.ifremer.fr/doc/00374/48484/48754.fasta https://archimer.ifremer.fr/doc/00374/48484/48755.csv https://archimer.ifremer.fr/doc/00374/48484/48756.csv https://archimer.ifremer.fr/doc/00374/48484/48757.csv https://archimer.ifremer.fr/doc/00374/48484/48758.csv https://archimer.ifremer.fr/doc/00374/48484/48759.csv LA English DT Article AB The king scallop Pecten maximus is a high valuable species of great interest in Europe for both fishery and aquaculture. Notably, there has been an increased investment to produce seed for enhancement programmes of wild scallop populations. However, hatchery production is a relatively new industry and it is still underdeveloped. Major hurdles are spawning control and gamete quality. In the present study, a total of 14 scallops were sampled in the bay of Brest (Brittany, France) to compare transcriptomic profiles of mature oocytes collected by spawning induction or by stripping. To reach such a goal, a microarray analysis was performed by using a custom 8x60K oligonucleotide microarray representing 45,488 unique scallop contigs. First we identified genes that were differentially expressed depending on oocyte quality, estimated as the potential to produce D-larvae. Secondly, we investigated the transcriptional features of both stripped and spawned oocytes. Genes coding for proteins involved in cytoskeletal dynamics, serine/threonine kinases signalling pathway, mRNA processing, response to DNA damage, apoptosis and cell-cycle appeared to be of crucial importance for both oocyte maturation and developmental competence. This study allowed us to dramatically increase the knowledge about transcriptional features of oocyte quality and maturation, as well as to propose for the first time putative molecular markers to solve a major bottleneck in scallop aquaculture. PY 2017 PD MAR SO Plos One SN 1932-6203 PU Public Library Science VL 12 IS 3 UT 000396011300035 DI 10.1371/journal.pone.0172805 ID 48484 ER EF