FN Archimer Export Format PT J TI Response of the pearl oyster Pinctada margaritifera to cadmium and chromium: Identification of molecular biomarkers BT AF GUEGUEN, Yannick SAULNIER, Denis SANTINI, Adrien MAGRE, Kevin GAREN, Pierre BERNAGOUT, Solene NOHL, Marine BOUISSET, Patrick HELME, Herehia PLANES, Serge LE MOULLAC, Gilles AS 1:1,2;2:1;3:1;4:1;5:1;6:3;7:3;8:3;9:3;10:4;11:1; FF 1:PDG-RBE-IHPE;2:PDG-RBE-RMPF;3:PDG-RBE-RMPF;4:PDG-RBE-RMPF;5:PDG-RBE-RMPF;6:;7:;8:;9:;10:;11:PDG-RBE-RMPF; C1 UPF, IFREMER, ILM IRD, UMR EIO 241, Labex Corail,BP 49, F-98719 Tahiti, French Polynesi, France. Univ Montpellier, UPVD, CNRS, IFREMER,IHPE UMR 5244, CC 80, F-34095 Montpellier, France. IRSN LESE, BP 182, F-98725 Tahiti, French Polynesi, France. EPHE, CRIOBE, USR CNRS 3278, Labex Corail, Moorea, French Polynesi, France. C2 IFREMER, FRANCE IFREMER, FRANCE IRSN, FRANCE EPHE, FRANCE SI MONTPELLIER TAHITI SE PDG-RBE-IHPE PDG-RBE-RMPF UM IHPE EIO IN WOS Ifremer jusqu'en 2018 copubli-france IF 3.241 TC 17 UR https://archimer.ifremer.fr/doc/00376/48707/49146.pdf LA English DT Article DE ;Pinctada margaritifera;French Polynesia;Cadmium;Chromium;Gene expression;Metallothionein AB This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10 μg L− 1) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues. In response to Cd-Cr contamination, metallothionein (MT) was significantly up-regulated by Cd-Cr at both concentrations, while 7 others (SOD, CAT, GPX, GSTO, GSTM, CASP, MDR) were down-regulated. Based on the development of these molecular tools, we propose that the pearl oyster, P. margaritifera, could be used as a sentinel species for heavy metal contamination in the lagoons of tropical ecosystems. PY 2017 PD MAY SO Marine Pollution Bulletin SN 0025-326X PU Pergamon-elsevier Science Ltd VL 118 IS 1-2 UT 000402217300063 BP 420 EP 426 DI 10.1016/j.marpolbul.2017.03.012 ID 48707 ER EF