FN Archimer Export Format PT J TI Inter and intra-specific growth and domoic acid production in relation to nutrient ratios and concentrations in Pseudo-nitzschia : phosphate an important factor BT AF LEMA, Anais Kimberley LATIMIER, Marie NEZAN, Elisabeth FAUCHOT, Juliette LE GAC, Mickael AS 1:1;2:1;3:2;4:3,4;5:1; FF 1:PDG-ODE-DYNECO-PELAGOS;2:PDG-ODE-DYNECO-PELAGOS;3:PDG-ODE-LITTORAL-LERBO;4:;5:PDG-ODE-DYNECO-PELAGOS; C1 IFREMER, DYNECO PELAGOS, F-29280 Plouzane, France. IFREMER, Stn Biol Marine, Pl Croix,BP 40537, F-29185 Concarneau, France. Normandie Univ, UNICAEN, F-14000 Caen, France. UCBN, UPMC, MNHN, CNRS 7208,IRD 207,UMR BORER, F-14032 Caen, France. C2 IFREMER, FRANCE IFREMER, FRANCE UNIV NORMANDIE, FRANCE CNRS, FRANCE SI BREST CONCARNEAU SE PDG-ODE-DYNECO-PELAGOS PDG-ODE-LITTORAL-LERBO IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 4.138 TC 24 UR https://archimer.ifremer.fr/doc/00377/48849/49397.pdf LA English DT Article CR COMOR 41 BO Thalia DE ;Pseudo-nitzschia strains;Domoic acid;Ratio;Phosphate;Nitrogen AB The factors responsible for inducing the synthesis of toxins and responses from toxic phytoplankton blooms remain unclear. In this study we compare the influence of genotypic (at both the intra and interspecific levels) and environmental factors (nutrient concentration and ratio) on growth (in terms of cell densities) and domoic acid (DA) production in three Pseudo-nitzschia species: P. australis, P.pungens and P.fradulenta. A strong phosphate effect was detected. More precisely, a low initial concentration in phosphate, even at high initial nitrogen and silicate concentrations, induced the highest DA concentrations and the lowest cell densities in all strains/species studied. In contrast, a low initial concentration of nitrogen and silicate combined, with a higher phosphate concentration resulted in low cell densities, but without high DA production. Inter-species effects were also observed in DA production, where P. australis represented the most toxigenic species of all. Intra-specific variations were only moderate, except for a recently isolated P. australis strain, suggesting the influence of time since isolation on the physiology and DA production of Pseudo-nitzschia species. Overall, the lack of strong interaction between environmental and genotypic factors showed that the various genotypes investigated did not extensively diverge in their ability to respond (in terms of DA production and cell densities) to contrasting nutrient supply. PY 2017 PD APR SO Harmful Algae SN 1568-9883 PU Elsevier Science Bv VL 64 UT 000400529900002 BP 11 EP 19 DI 10.1016/j.hal.2017.03.001 ID 48849 ER EF