FN Archimer Export Format
PT J
TI Sensitivity of eastern oyster ( Crassostrea virginica) spermatozoa and oocytes to dispersed oil: Cellular responses and impacts on fertilization and embryogenesis
BT 
AF VIGNIER, J.
   VOLETY, A. K.
   ROLTON, A.
   LE GOIC, N.
   CHU, F. -L. E.
   ROBERT, Rene
   SOUDANT, P.
AS 1:1,2;2:1,5;3:1,2;4:2;5:3;6:4;7:2;
FF 1:;2:;3:;4:;5:;6:PDG-ODE-LITTORAL;7:;
C1 Florida Gulf Coast Univ, Coll Arts & Sci, Dept Marine & Ecol Sci, Ft Myers, FL 33965 ,USA.
   IUEM UBO, Lab Sci Environm Marin, UMR LEMAR 6539, Technopole Brest Iroise, F-29280 Plouzane, France.
   Virginia Inst Marine Sci, Coll William & Mary, Dept Aquat Hlth Sci, Gloucester Point, VA 23062, USA.
   IFREMER, Unite Littoral, Ctr Bretagne, CS 10070, F-29280 Plouzane, France.
   Univ North Carolina Wilmington, 601S Coll Rd, Wilmington, NC 28403, USA.
C2 UNIV FLORIDA GULF COAST, USA
   UBO, FRANCE
   VIRGINIA INST MARINE SCI, USA
   IFREMER, FRANCE
   UNIV NORTH CAROLINA WILMINGTON, USA
SI BREST
SE PDG-ODE-LITTORAL
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 4.358
TC 23
UR https://archimer.ifremer.fr/doc/00377/48851/51168.pdf
LA English
DT Article
DE ;Deepwater Horizon oil spill;Oyster;Spermatozoa;Oocyte;Flow-cytometry
AB The 2010 Deepwater Horizon (DWH) oil spill released millions of barrels of oil and dispersant into the Gulf of Mexico. The timing of the spill coincided with the spawning season of Crassostrea virginica. Consequently, gametes released in the water were likely exposed to oil and dispersant. This study aimed to (i) evaluate the cellular effects of acute exposure of spermatozoa and oocytes to surface slick oil, dispersed mechanically (HEWAF) and chemically (CEWAF), using flow-cytometric (FCM) analyses, and (ii) determine whether the observed cellular effects relate to impairments of fertilization and embryogenesis of gametes exposed to the same concentrations of CEWAF and HEWAF. Following a 30-min exposure, the number of spermatozoa and their viability were reduced due to a physical action of oil droplets (HEWAF) and a toxic action of CEWAF respectively. Additionally, reactive oxygen species (ROS) production in exposed oocytes tended to increase with increasing oil concentrations suggesting that exposure to dispersed oil resulted in an oxidative stress. The decrease in fertilization success (1-h), larval survival (24-h) and increase in abnormalities (6-h and 24-h) may be partly related to altered cellular characteristics. FCM assays are a good predictor of sublethal effects especially on fertilization success. These data suggest that oil/dispersant are cytotoxic to gametes, which may affect negatively the reproduction success and early development of oysters. 
PY 2017
PD JUL
SO Environmental Pollution
SN 0269-7491
PU Elsevier Sci Ltd
VL 225
UT 000401679200029
BP 270
EP 282
DI 10.1016/j.envpol.2016.11.052
ID 48851
ER

EF