FN Archimer Export Format
PT J
TI Structural Iron (II) of Basaltic Glass as an Energy Source for Zetaproteobacteria in an Abyssal Plain Environment, Off the Mid Atlantic Ridge
BT 
AF HENRI, Pauline A.
   ROMMEVAUX-JESTIN, Celine
   LESONGEUR, Francoise
   MUMFORD, Adam
   EMERSON, David
   GODFROY, Anne
   MENEZ, Benedicte
AS 1:1;2:1;3:2;4:3;5:3;6:2;7:1;
FF 1:;2:;3:PDG-REM-EEP-LMEE;4:;5:;6:PDG-REM-EEP-LMEE;7:;
C1 Univ Paris Diderot, Sorbonne Paris Cite, Inst Phys Globe Paris, CNRS, Paris, France.
   CNRS, UMR 6197, Lab Microbiol Environm Extremes, Plouzane, France.
   Bigelow Lab Ocean Sci, East Boothbay, ME USA.
C2 UNIV PARIS 07, FRANCE
   IFREMER, FRANCE
   BIGELOW LAB OCEAN SCI, USA
SI BREST
SE PDG-REM-EEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-int-hors-europe
IF 4.076
TC 29
UR https://archimer.ifremer.fr/doc/00378/48894/49332.pdf
   https://archimer.ifremer.fr/doc/00378/48894/49333.pdf
LA English
DT Article
CR GRAVILUCK
   MOMARDREAM-NAUT1-NAUT2
   MOMARSAT : MONITORING THE MID ATLANTIC RIDGE
BO L'Atalante
   Pourquoi pas ?
DE ;basaltic glass;Zetaproteobacteria;bio-mediated alteration;iron-oxidation;abyssal plain
AB To explore the capability of basaltic glass to support the growth of chemosynthetic microorganisms, complementary in situ and in vitro colonization experiments were performed. Microbial colonizers containing synthetic tholeitic basaltic glasses, either enriched in reduced or oxidized iron, were deployed off-axis from the Mid Atlantic Ridge on surface sediments of the abyssal plain (35°N; 29°W). In situ microbial colonization was assessed by sequencing of the 16S rRNA gene and basaltic glass alteration was characterized using Scanning Electron Microscopy, micro-X-ray Absorption Near Edge Structure at the Fe-K-edge and Raman microspectroscopy. The colonized surface of the reduced basaltic glass was covered by a rind of alteration made of iron-oxides trapped in a palagonite-like structure with thicknesses up to 150 μm. The relative abundance of the associated microbial community was dominated (39% of all reads) by a single operational taxonomic unit (OTU) that shared 92% identity with the iron-oxidizer Mariprofundus ferrooxydans PV-1. Conversely, the oxidized basaltic glass showed the absence of iron-oxides enriched surface deposits and correspondingly there was a lack of known iron-oxidizing bacteria in the inventoried diversity. In vitro, a similar reduced basaltic glass was incubated in artificial seawater with a pure culture of the iron-oxidizing M. ferrooxydans DIS-1 for 2 weeks, without any additional nutrients or minerals. Confocal Laser Scanning Microscopy revealed that the glass surface was covered by twisted stalks characteristic of this iron-oxidizing Zetaproteobacteria. This result supported findings of the in situ experiments indicating that the Fe(II) present in the basalt was the energy source for the growth of representatives of Zetaproteobacteria in both the abyssal plain and the in vitro experiment. In accordance, the surface alteration rind observed on the reduced basaltic glass incubated in situ could at least partly result from their activity. 
PY 2016
PD JAN
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media Sa
VL 6
IS 1518
UT 000368387300001
BP 1
EP 18
DI 10.3389/fmicb.2015.01518
ID 48894
ER

EF