FN Archimer Export Format
PT J
TI Marine green macroalgae: a source of natural compounds with mineralogenic and antioxidant activities
BT 
AF SURGET, Gwladys
   ROBERTO, Vania P.
   LE LANN, Klervi
   MIRA, Sara
   GUERARD, Fabienne
   LAIZE, Vincent
   POUPART, Nathalie
   LEONOR CANCELA, M.
   STIGER-POUVREAU, Valerie
AS 1:1;2:2;3:1;4:2;5:1;6:2;7:1;8:2,3;9:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;
C1 Univ Brest, CNRS, IRD, Ifremer,LEMAR,UMR 6539,IUEM, F-29280 Plouzane, France.
   Univ Algarve, Ctr Marine Sci CCMAR, Faro, Portugal.
   Univ Algarve, Dept Biomed Sci & Med DCBM, Faro, Portugal.
C2 UBO, FRANCE
   UNIV ALGARVE, PORTUGAL
   UNIV ALGARVE, PORTUGAL
UM LEMAR
IF 2.401
TC 38
UR https://archimer.ifremer.fr/doc/00381/49282/49839.pdf
LA English
DT Article
DE ;Ulvophyceae;Phenolic compounds;Antioxidant activity;Mineralogenic activity;Proliferative activity
AB Marine macroalgae represent a valuable natural resource for bioactive phytochemicals with promising applications in therapeutics, although they remain largely under-exploited. In this work, the potential of two marine green macroalgae (Cladophora rupestris and Codium fragile) as a source of bioactive phenolic compounds was explored, and antioxidant, mineralogenic, and osteogenic activities were evaluated. For each species, a crude hydroalcoholic extract (CE) was prepared by solid/liquid extraction and fractionated by liquid/liquid purification into an ethyl acetate fraction (EAF) enriched in phenolic compounds and an aqueous fraction (AF). Antioxidant activity, assessed through radical scavenging activity and reducing power assay, was increased in EAF fraction of both species and closely related to the phenolic content in each fraction. Mineralogenic activity, assessed through extracellular matrix mineralization of a fish bone-derived cell line, was induced by EAF fractions (up to 600 % for C. rupestris EAF). Quantitative analysis of operculum formation in zebrafish larvae stained with alizarin red S further confirmed the osteogenic potential of EAF fractions in vivo, with an increase of more than 1.5-fold for both C. fragile and C. rupestris fractions, similar to vitamin D (control). Our results demonstrated a positive correlation between phenolic fractions and biological activity, suggesting that phenolic compounds extracted from marine green macroalgae may represent promising molecules toward therapeutic applications in the field of bone biology. 
PY 2017
PD FEB
SO Journal Of Applied Phycology
SN 0921-8971
PU Springer
VL 29
IS 1
UT 000396344800051
BP 575
EP 584
DI 10.1007/s10811-016-0968-3
ID 49282
ER

EF