FN Archimer Export Format
PT J
TI Modelization of the regulation of protein synthesis following fertilization in sea urchin shows requirement of two processes: a destabilization of elF4E:4E-BP complex and a great stimulation of the 4E-BP-degradation mechanism, both rapamycin-sensitive
BT 
AF LAURENT, Sebastien
   RICHARD, Adrien
   MULNER-LORILLON, Odile
   MORALES, Julia
   FLAMENT, Didier
   GLIPPA, Virginie
   BOURDON, Jeremie
   GOSSELIN, Pauline
   SIEGEL, Anne
   CORMIER, Patrick
   BELLE, Robert
AS 1:1;2:2;3:3,4;4:3,4;5:1;6:3,4;7:5;8:3,4;9:6,7;10:3,4;11:3,4;
FF 1:PDG-REM-EEP-LMEE;2:;3:;4:;5:PDG-REM-EEP-LMEE;6:;7:;8:;9:;10:;11:;
C1 IFREMER, Lab Microbiol Environm Extremes, UMR6197, Plouzane, France.
   Univ Nice Sophia Antipolis, Lab I3S, UMR 7271, Sophia, Antipolis, France.
   UPMC, Univ Sorbonne, UMR 8227, Stn Biol Roscoff, F-29688 Roscoff, France.
   CNRS, UMR 8227, Stn Biol Roscoff, F-29688 Roscoff, France.
   Univ Nantes, CNRS, Lab LINA, UMR 6241, Nantes, France.
   CNRS, IRISA, UMR 6074, Rennes, France.
   INRIA, Ctr Rennes Bretagne Atlantic, Rennes, France.
C2 IFREMER, FRANCE
   UNIV NICE, FRANCE
   UNIV PARIS 06, FRANCE
   CNRS, FRANCE
   UNIV NANTES, FRANCE
   CNRS, FRANCE
   INRIA, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.789
TC 5
UR https://archimer.ifremer.fr/doc/00382/49341/49731.pdf
LA English
DT Article
DE ;translational control;sea urchin embryos;mechanisms of fertilization;deterministic model;translation simulation
AB Fertilization of sea urchin eggs involves an increase in protein synthesis associated with a decrease in the amount of the translation initiation inhibitor 4E-BP A highly simple reaction model for the regulation of protein synthesis was built and was used to simulate the physiological changes in the total 4E-BP amount observed during time after fertilization. Our study evidenced that two changes occurring at fertilization are necessary to fit with experimental data. The first change was an 8-fold increase in the dissociation parameter (koffi) of the elF4E:4E-BP complex. The second was an important 32.5-fold activation of the degradation mechanism of the protein 4E-BP Additionally, the changes in both processes should occur in 5 min time interval post-fertilization. To validate the model, we checked that the kinetic of the predicted 4.2-fold increase of elF4E:e1F4G complex concentration at fertilization matched the increase of protein synthesis experimentally observed after fertilization (6.6-fold, SD = 2.3, n = 8). The minimal model was also used to simulate changes observed after fertilization in the presence of rapamycin, a FRAP/mTOR inhibitor. The model showed that the elF4E:4E-BP complex destabilization was impacted and surprisingly, that the mechanism of 4E-BP degradation was also strongly affected, therefore suggesting that both processes are controlled by the protein kinase FRAP/mTOR. 
PY 2014
PD MAY
SO Frontiers In Genetics
SN 1664-8021
PU Frontiers Media Sa
VL 5
IS 117
UT 000347056800001
BP 1
EP 10
DI 10.3389/fgene.2014.00117
ID 49341
ER

EF