FN Archimer Export Format
PT J
TI Sexually dimorphic gene expressions in eels: useful markers for early sex assessment in a conservation context
BT 
AF GEFFROY, Benjamin
   GUILBAUD, Florian
   AMILHAT, Elsa
   BEAULATON, Laurent
   VIGNON, Matthias
   HUCHET, Emmanuel
   RIVES, Jacques
   BOBE, Julien
   FOSTIER, Alexis
   GUIGUEN, Yann
   BARDONNET, Agnes
AS 1:1,2,3;2:1;3:4;4:5,6;5:2,3;6:2,3;7:2,3;8:1;9:1;10:1;11:2,3;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;
C1 INRA, LPGP UR1037, Fish Physiol & Genom, Campus Beaulieu, F-35000 Rennes, France.
   INRA, UMR Ecobiop 1224, Aquapole, Pole GestAqua, F-64310 Quartier Ibarron, St Pee Sur Nive, France.
   UPPA, UMR Ecobiop 1224, UFR Sci Cote Basque, Allee Parc Montaury, F-64600 Anglet, France.
   Univ Perpignan, UMR CNRS UPVD CEFREM 5110, Batiment R,58 Ave Paul Alduy, F-66860 Perpignan, France.
   Onema, Pole GestAqua, 65 Rue St Brieuc, F-35042 Rennes, France.
   INRA, Pole GestAqua, U3E 1224, 65 Rue St Brieuc, F-35042 Rennes, France.
C2 INRA, FRANCE
   INRA, FRANCE
   UNIV PAU & PAYS ADOUR, FRANCE
   UNIV PERPIGNAN, FRANCE
   ONEMA, FRANCE
   INRA, FRANCE
IN DOAJ
IF 4.259
TC 23
UR https://archimer.ifremer.fr/doc/00386/49723/50254.pdf
LA English
DT Article
DE ;Conservation biology;Ecology;Ecophysiology;Transcription
AB Environmental sex determination (ESD) has been detected in a range of vertebrate reptile and fish species. Eels are characterized by an ESD that occurs relatively late, since sex cannot be histologically determined before individuals reach 28 cm. Because several eel species are at risk of extinction, assessing sex at the earliest stage is a crucial management issue. Based on preliminary results of RNA sequencing, we targeted genes susceptible to be differentially expressed between ovaries and testis at different stages of development. Using qPCR, we detected testis-specific expressions of dmrt1, amh, gsdf and pre-miR202 and ovary-specific expressions were obtained for zar1, zp3 and foxn5. We showed that gene expressions in the gonad of intersexual eels were quite similar to those of males, supporting the idea that intersexual eels represent a transitional stage towards testicular differentiation. To assess whether these genes would be effective early molecular markers, we sampled juvenile eels in two locations with highly skewed sex ratios. The combined expression of six of these genes allowed the discrimination of groups according to their potential future sex and thus this appears to be a useful tool to estimate sex ratios of undifferentiated juvenile eels. 
PY 2016
PD SEP
SO Scientific Reports
SN 2045-2322
PU Nature Publishing Group
VL 6
IS 34041
UT 000384047000001
BP 1
EP 11
DI 10.1038/srep34041
ID 49723
ER

EF