Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas

Type Article
Date 2017-05
Language English
Author(s) Travers Marie-AgnesORCID1, Tourbiez Delphine1, Parizadeh Leila1, Haffner Philippe1, 2, Kozic-Djellouli Angelique1, Aboubaker Mohamed3, Koken Marcel4, Degremont LionelORCID1, Lupo CoralieORCID1
Affiliation(s) 1 : IFREMER, LGPMM SG2M, Lab Genet & Pathol Mollusques Marins, Ave Mus de Loup, F-17390 La Tremblade, France.
2 : Univ Montpellier, IHPE, CNRS, UMR 5244,IFREMER, Univ Perpignan Via Domitia, F-34095 Montpellier, France.
3 : BP 1119, Djibouti, Djibouti.
4 : LABOCEA CNRS, 120 Ave Alexis de Rochon, F-29280 Plouzane, France.
Source Veterinary Research (0928-4249) (Biomed Central Ltd), 2017-05 , Vol. 48 , N. 32 , P. 1-8
DOI 10.1186/s13567-017-0438-1
WOS© Times Cited 2
Abstract

This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population.

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Travers Marie-Agnes, Tourbiez Delphine, Parizadeh Leila, Haffner Philippe, Kozic-Djellouli Angelique, Aboubaker Mohamed, Koken Marcel, Degremont Lionel, Lupo Coralie (2017). Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas. Veterinary Research, 48(32), 1-8. Publisher's official version : https://doi.org/10.1186/s13567-017-0438-1 , Open Access version : https://archimer.ifremer.fr/doc/00386/49768/