FN Archimer Export Format
PT J
TI Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas
BT 
AF TRAVERS, Marie-Agnes
   TOURBIEZ, Delphine
   PARIZADEH, Leila
   HAFFNER, Philippe
   KOZIC-DJELLOULI, Angelique
   ABOUBAKER, Mohamed
   KOKEN, Marcel
   DEGREMONT, Lionel
   LUPO, Coralie
AS 1:1;2:1;3:1;4:1,2;5:1;6:3;7:4;8:1;9:1;
FF 1:PDG-RBE-SG2M-LGPMM;2:PDG-RBE-SG2M-LGPMM;3:PDG-RBE-SG2M-LGPMM;4:PDG-RBE-IHPE;5:PDG-RBE-SG2M-LGPMM;6:;7:;8:PDG-RBE-SG2M-LGPMM;9:PDG-RBE-SG2M-LGPMM;
C1 IFREMER, LGPMM SG2M, Lab Genet & Pathol Mollusques Marins, Ave Mus de Loup, F-17390 La Tremblade, France.
   Univ Montpellier, IHPE, CNRS, UMR 5244,IFREMER, Univ Perpignan Via Domitia, F-34095 Montpellier, France.
   BP 1119, Djibouti, Djibouti.
   LABOCEA CNRS, 120 Ave Alexis de Rochon, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
   BP 1119, Djibouti, Djibouti.
   LABOCEA CNRS, FRANCE
SI LA TREMBLADE
   MONTPELLIER
   NANTES
SE PDG-RBE-SG2M-LGPMM
   PDG-RBE-IHPE
UM IHPE
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 2.903
TC 17
UR https://archimer.ifremer.fr/doc/00386/49768/50308.pdf
LA English
DT Article
AB This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. 
PY 2017
PD MAY
SO Veterinary Research
SN 0928-4249
PU Biomed Central Ltd
VL 48
IS 32
UT 000402333400001
BP 1
EP 8
DI 10.1186/s13567-017-0438-1
ID 49768
ER

EF