FN Archimer Export Format
PT J
TI ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas )
BT 
AF BOULAIS, Myrina
   SOUDANT, Philippe
   LE GOIC, Nelly
   QUERE, Claudie
   BOUDRY, Pierre
   SUQUET, Marc
AS 1:1;2:2;3:2;4:3;5:3;6:1;
FF 1:PDG-RBE-PFOM-PI;2:;3:;4:PDG-RBE-PFOM-PI;5:PDG-RBE-PFOM;6:PDG-RBE-PFOM-PI;
C1 UBO, IFREMER, CNRS, UMR 6539,LEMAR,IRD, Site Expt Argenton, Landunvez, France.
   Univ Bretagne Occidentale, IFREMER, CNRS, IUEM,UMR 6539,LEMAR,IRD, Plouzane, France.
   UBO, IFREMER, Ctr Bretagne, UMR 6539,LEMAR,CNRS,IRD, Plouzane, France.
C2 IFREMER, FRANCE
   UBO, FRANCE
   IFREMER, FRANCE
SI BREST
   ARGENTON
SE PDG-RBE-PFOM-PI
   PDG-RBE-PFOM
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.71
TC 19
UR https://archimer.ifremer.fr/doc/00386/49769/50309.pdf
LA English
DT Article
DE ;Spermatozoa;Quality;Motility;Viability;ATP content;ROS production
AB Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability of fertilizing ability demonstrated using simple and multiple regressions. Our results showed that ATP content depends on both MMP and viability of spermatozoa. Using multiple regressions, 61% of the variability of the trochophore-larval yield was explained by a model combining the ATP content and the percentage of dying spermatozoa (P < 0.001). Our results reveal that capacity of spermatozoa to maintain a high level of ATP via OXPHOS partly explains the inter-individual variability of fertilization success in the Pacific oyster. Sperm ATP content and viability assays will provide valuable tools for assessing sperm quality of this species in aquaculture production, cryopreservation, and bioassays. 
PY 2017
PD OCT
SO Aquaculture
SN 0044-8486
PU Elsevier Science Bv
VL 479
UT 000408034700015
BP 114
EP 119
DI 10.1016/j.aquaculture.2017.05.035
ID 49769
ER

EF