FN Archimer Export Format PT J TI Gene-centromere mapping in meiotic gynogenetic European seabass BT AF ORAL, Munevver COLLETER, Julie BEKAERT, Mich TAGGART, John B. PALAIOKOSTAS, Christos MCANDREW, Brendan J. VANDEPUTTE, Marc CHATAIN, Beatrice KUHL, Heiner REINHARDT, Richard PERUZZI, Stefano PENMAN, David J. AS 1:1;2:2,3;3:1;4:1;5:1;6:1;7:3,4;8:3;9:5;10:6;11:7;12:1; FF 1:;2:;3:;4:;5:;6:;7:;8:PDG-RBE-MARBEC-L3AS;9:;10:;11:;12:; C1 Univ Stirling, Inst Aquaculture, Sch Nat Sci, Stirling FK9 4LA, Scotland. Cirad, Persyst, UMR Intrepid, Campus Int Baillarguet, F-34398 Montpellier, France. IFREMER, F-34250 Palavas Les Flots, France. Univ Paris Saclay, INRA, GABI, AgroParisTech, F-78350 Jouy En Josas, France. Leibniz Inst Freshwater Biol & Inland Fisheries, Muggelseedamm 310, D-12587 Berlin, Germany. Max Planck Genome Ctr Cologne, Max Planck Inst Plant Breeding, Carl von LinnA Weg 10, D-50829 Cologne, Germany. Univ Tromso, Fac Biosci Fisheries & Econ, Dept Arctic & Marine Biol, N-9037 Tromso, Norway. C2 UNIV STIRLING, UK CIRAD, FRANCE IFREMER, FRANCE INRA, FRANCE IGB, GERMANY MAX PLANCK INST, GERMANY UNIV TROMSO, NORWAY SI PALAVAS SE PDG-RBE-MARBEC-L3AS UM MARBEC IN WOS Ifremer jusqu'en 2018 DOAJ copubli-france copubli-p187 copubli-europe IF 3.73 TC 8 UR https://archimer.ifremer.fr/doc/00388/49901/50462.pdf https://archimer.ifremer.fr/doc/00388/49901/50463.csv https://archimer.ifremer.fr/doc/00388/49901/50464.csv https://archimer.ifremer.fr/doc/00388/49901/50465.csv https://archimer.ifremer.fr/doc/00388/49901/50466.csv https://archimer.ifremer.fr/doc/00388/49901/50467.png https://archimer.ifremer.fr/doc/00388/49901/50468.fasta https://archimer.ifremer.fr/doc/00388/49901/50469.csv https://archimer.ifremer.fr/doc/00388/49901/50470.csv https://archimer.ifremer.fr/doc/00388/49901/50471.csv LA English DT Article DE ;Dicentrarchus labrax;Meiotic gynogenesis;Isogenic lines;ddRAD seq;Genetic map;Gene-Centromere map;Aquaculture AB Background Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families. Results From a single meiotic gynogenetic family consisting of 79 progeny, 42 million sequencing reads (Illumina, trimmed to 148 bases) resolved 6866 unique RAD-tags. The 340 male-informative SNP markers that were identified confirmed the lack of paternal contribution. A gene-centromere map was constructed based on 804 female-informative SNPs in 24 linkage groups (2n = 48) with a total length of 1251.02 cM (initial LG assignment was based on the seabass genome assembly, dicLab v1). Chromosome arm structure could be clearly discerned from the pattern of heterozygosity in each linkage group in 18 out of 24 LGs: the other six showed anomalies that appeared to be related to issues in the genome assembly. Conclusion Genome-wide screening enabled substantive verification of the production of the gynogenetic family used in this study. The large number of telomeric and subtelomeric markers with high heterozygosity values in the meiotic gynogenetic family indicate that such markers could be used to clearly distinguish between meiotic and mitotic gynogenetics. PY 2017 PD JUL SO Bmc Genomics SN 1471-2164 PU Biomed Central Ltd VL 18 IS 1 UT 000404080900001 DI 10.1186/s12864-017-3826-z ID 49901 ER EF