A multistrain approach to studying the mechanisms underlying compatibility in the interaction between Biomphalaria glabrata and Schistosoma mansoni

Type Article
Date 2017-03
Language English
Author(s) Galinier Richard1, Roger Emmanuel1, Mone Yves1, Duval David1, Portet Anais1, Pinaud Silvain1, Chaparro Cristian1, Grunau Christoph1, Genthon Clemence2, Dubois Emeric2, Rognon Anne1, Arancibia Nathalie1, Dejean Bernard1, Theron Andre1, Gourbal Benjamin1, Mitta GuillaumeORCID
Affiliation(s) 1 : Univ Montpellier, Univ Perpignan, CNRS, IFREMER,IHPE UMR 5244, Via Domitia, Perpignan, France.
2 : Montpellier Genom & Bioinformat Facil, MGX Montpellier GenomiX, Montpellier, France.
Source Plos Neglected Tropical Diseases (1935-2735) (Public Library Science), 2017-03 , Vol. 11 , N. 3 , P. e0005398 (1-25)
DOI 10.1371/journal.pntd.0005398
WOS© Times Cited 28

In recent decades, numerous studies have sought to better understand the mechanisms underlying the compatibility between Biomphalaria glabrata and Schistosoma mansoni. The developments of comparative transcriptomics, comparative genomics, interactomics and more targeted approaches have enabled researchers to identify a series of candidate genes. However, no molecular comparative work has yet been performed on multiple populations displaying different levels of compatibility. Here, we seek to fill this gap in the literature. We focused on B. glabrata FREPs and S. mansoni SmPoMucs, which were previously demonstrated to be involved in snail/schistosome compatibility. We studied the expression and polymorphisms of these factors in combinations of snail and schistosome isolates that display different levels of compatibility. We found that the polymorphism and expression levels of FREPs and SmPoMucs could be linked to the compatibility level of S. mansoni. These data and our complementary results obtained by RNA-seq of samples from various snail strains indicate that the mechanism of compatibility is much more complex than previously thought, and that it is likely to be highly variable within and between populations. This complexity must be taken into account if we hope to identify the molecular pathways that are most likely to be good targets for strategies aimed at blocking transmission of the parasite through the snail intermediate host.

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Publisher's official version 25 4 MB Open access
S1 Fig. Validation of FREP by alignment of sequences obtained from Sanger sequencing and from computational assembly of de novo transcripts. 4 75 KB Open access
S1 Table. Variants of cDNA obtained from 11 individual sporocysts of S. mansoni strains. 346 KB Open access
S2 Table. B glabrata immunome 15 KB Open access
S3 Table. Annotation of the 24 classes of FREP molecules identified. 13 KB Open access
S4 Table. Transcriptome statistics. 10 KB Open access
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Galinier Richard, Roger Emmanuel, Mone Yves, Duval David, Portet Anais, Pinaud Silvain, Chaparro Cristian, Grunau Christoph, Genthon Clemence, Dubois Emeric, Rognon Anne, Arancibia Nathalie, Dejean Bernard, Theron Andre, Gourbal Benjamin, Mitta Guillaume (2017). A multistrain approach to studying the mechanisms underlying compatibility in the interaction between Biomphalaria glabrata and Schistosoma mansoni. Plos Neglected Tropical Diseases, 11(3), e0005398 (1-25). Publisher's official version : https://doi.org/10.1371/journal.pntd.0005398 , Open Access version : https://archimer.ifremer.fr/doc/00388/49951/