FN Archimer Export Format
PT J
TI Antimelanoma activity of Heterocapsa triquetra pigments
BT 
AF HAGUET, Quentin
   BONNET, Antoine
   BERARD, Jean-Baptiste
   GOLDBERG, Joelle
   JOGUET, Nicolas
   FLEURY, Audrey
   THIERY, Valerie
   PICOT, Laurent
AS 1:1;2:1,2;3:3;4:1;5:4;6:1;7:1;8:1;
FF 1:;2:;3:PDG-RBE-BRM-PBA;4:;5:;6:;7:;8:;
C1 Univ La Rochelle, UMRi CNRS LIENSs 7266, F-17042 La Rochelle, France.
   Univ La Rochelle, Plateforme Anal Haute Resolut Biomol, UMRi CNRS LIENSs 7266, F-17042 La Rochelle, France.
   IFREMER, Lab BRM PBA, Rue Ile dYeu, F-44311 Nantes, France.
   IDCAPS, Filiale R&D INNOVIA, ZI Chef Baie, 4 Rue Samuel Champlain, F-17000 La Rochelle, France.
C2 UNIV LA ROCHELLE, FRANCE
   UNIV LA ROCHELLE, FRANCE
   IFREMER, FRANCE
   IDCAPS, FRANCE
SI NANTES
SE PDG-RBE-BRM-PBA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.745
TC 10
UR https://archimer.ifremer.fr/doc/00390/50127/50806.pdf
LA English
DT Article
DE ;Dinophyte;Heterocapsa;Melanoma;Microalgae;Microwave assisted extraction;Pigment
AB Heterocapsa triquetra (Ht), a non-toxic dinophyte, was selected to investigate the presence of pigments inhibiting melanoma cells growth. We developed an innovative microwave-assisted extraction process (MAE), determined the Ht pigment composition using Reverse Phase-High Performance Liquid Chromatography (HPLC) and high-resolution Ultra Performance Liquid Chromatography-Mass Spectrometry E (UPLC-MSE) analysis and assessed their antiproliferative activity in human invasive melanoma cells (A2058) grown in vitro. MAE allowed a fast and efficient extraction of Ht pigments as compared with conventional processes (soaking, sonication). Seventeen pigments and derivatives were unequivocally identified by UPLC-MSᴱ in the Ht extract, among which two were supposed to be produced during the extraction (hydroxylated chlorophyll a and pheophytin a). Pigment composition was fully coherent with that usually described for the DINO-1 peridiniales order. It included the uncommon carotenoids P457, peridininol, peridinin, pyrrhoxanthin, diadinochrome and diadinoxanthin. Ht pigment ethanol extract induced 18% growth inhibition of A2058 malignant melanoma cell line after a 72 h treatment at 100 μg·mL− 1 and pigment purification confirmed the highly significant antiproliferative activity of chlorophyll c2, peridinin, dinoxanthin and diatoxanthin on this cell line. Our data demonstrate for the first time the effectiveness of microwave irradiation to extract pigments from dinophytes. They also indicate that Heterocapsa triquetra and other dinophytes are interesting sources to purify bioactive pigments with antiproliferative activities in chemoresistant melanoma cells. These results strongly support previous findings about the anticancer activity of phytoplankton pigments and reinforce the possible interest of these molecules as natural cytostatic drugs. 
PY 2017
PD JUN
SO Algal Research-biomass Biofuels And Bioproducts
SN 2211-9264
PU Elsevier Science Bv
VL 25
UT 000406305900025
BP 207
EP 215
DI 10.1016/j.algal.2017.04.034
ID 50127
ER

EF