FN Archimer Export Format
PT J
TI The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway
BT 
AF ABIKHALIL, Celina
   FINKELSTEIN, Darren S.
   CONEJERO, Genevieve
   DU BOIS, Justin
   DESTOUMIEUX-GARZON, Delphine
   ROLLAND, Jean-Luc
AS 1:1;2:2;3:3,4;4:2;5:1;6:1;
FF 1:;2:;3:;4:;5:;6:PDG-RBE-IHPE;
C1 Univ Montpellier, IFREMER, CNRS, IHPE,UMR 5244,Univ Perpignan, Via Domitia, F-34095 Montpellier, France.
   Stanford Univ, Dept Chem, 333 Campus Dr, Stanford, CA 94305 USA.
   UMR B, Plant Cell Imaging Platform PHIV, Montpellier, France.
   UM, PMP, INRA, CNRS,SupAgro, Montpellier, France.
C2 IFREMER, FRANCE
   UNIV STANFORD, USA
   UNIV MONTPELLIER, FRANCE
   INRA, FRANCE
SI MONTPELLIER
SE PDG-RBE-IHPE
UM IHPE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
   copubli-int-hors-europe
IF 3.884
TC 27
UR https://archimer.ifremer.fr/doc/00391/50272/50905.pdf
LA English
DT Article
DE ;Shellfish;Toxin;Harmful algae;Phytoplankton
AB Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which is present in high concentrations in A. catenella, was found to be more toxic than saxitoxin (STX) to oyster immune cells. Altogether, results show that PSTs produced by toxic dinoflagellates enter the cytoplasm and induce apoptosis of oyster immune cells through a caspase-dependent pathway. Because of the central role of hemocytes in mollusc immune defense, PST-induced death of hemocytes could negatively affect resistance of bivalve molluscs to microbial infection. 
PY 2017
PD SEP
SO Aquatic Toxicology
SN 0166-445X
PU Elsevier Science Bv
VL 190
UT 000408783600016
BP 133
EP 141
DI 10.1016/j.aquatox.2017.07.001
ID 50272
ER

EF