Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures

Type Article
Date 2017-09
Language English
Author(s) Saulnier Denis1, de Decker Sophie2, Tourbiez Delphine2, Travers Marie-AgnesORCID2
Affiliation(s) 1 : IFREMER, UMR EIO 241, UPF ILM IRD, BP 49, F-98719 Tahiti, French Polynesi, France.
2 : IFREMER, RBE SG2M, Lab Genet & Pathol Mollusques Marins, Av Mus de Loup, F-17390 La Tremblade, French Polynesi, France.
Source Journal Of Microbiological Methods (0167-7012) (Elsevier Science Bv), 2017-09 , Vol. 140 , P. 67-69
DOI 10.1016/j.mimet.2017.07.002
WOS© Times Cited 15
Keyword(s) Multiplex real-time PCR, Gene sequencing, Molluscs, Splendidus Glade, Vibrio aestuarianus
Abstract

To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.

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Saulnier Denis, de Decker Sophie, Tourbiez Delphine, Travers Marie-Agnes (2017). Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures. Journal Of Microbiological Methods, 140, 67-69. Publisher's official version : https://doi.org/10.1016/j.mimet.2017.07.002 , Open Access version : https://archimer.ifremer.fr/doc/00392/50308/