FN Archimer Export Format PT J TI Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures BT AF SAULNIER, Denis DE DECKER, Sophie TOURBIEZ, Delphine TRAVERS, Marie-Agnes AS 1:1;2:2;3:2;4:2; FF 1:PDG-RBE-RMPF;2:;3:PDG-RBE-SG2M-LGPMM;4:PDG-RBE-SG2M-LGPMM; C1 IFREMER, UMR EIO 241, UPF ILM IRD, BP 49, F-98719 Tahiti, French Polynesi, France. IFREMER, RBE SG2M, Lab Genet & Pathol Mollusques Marins, Av Mus de Loup, F-17390 La Tremblade, French Polynesi, France. C2 IFREMER, FRANCE IFREMER, FRANCE SI TAHITI LA TREMBLADE SE PDG-RBE-RMPF PDG-RBE-SG2M-LGPMM UM EIO IN WOS Ifremer jusqu'en 2018 IF 1.701 TC 23 UR https://archimer.ifremer.fr/doc/00392/50308/51077.pdf LA English DT Article DE ;Multiplex real-time PCR;Gene sequencing;Molluscs;Splendidus Glade;Vibrio aestuarianus AB To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies. PY 2017 PD SEP SO Journal Of Microbiological Methods SN 0167-7012 PU Elsevier Science Bv VL 140 UT 000407982100011 BP 67 EP 69 DI 10.1016/j.mimet.2017.07.002 ID 50308 ER EF