Redescription and phylogenetic analyses of Durchoniella spp. (Ciliophora, Astomatida) associated with the polychaete Cirriformia tentaculata (Montagu, 1808)

Type Article
Date 2017-10
Language English
Author(s) Sauvadet Anne-Laure1, Lynn Denis H.2, 6, Roussel ErwanORCID3, Le Panse Sophie4, Bigeard Estelle1, Schrevel Joseph5, Guillou Laure1
Affiliation(s) 1 : Univ Pierre & Marie Curie Paris 6, Sorbonne Univ, CNRS, UMR 7144,Stn Biol Roscoff, Pl Georges Teissier,CS90074, F-29688 Roscoff, France.
2 : Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada.
3 : Cardiff Univ, Sch Earth & Ocean Sci, Main Bldg,Pk Pl, Cardiff, S Glam, Wales.
4 : CNRS, FR 2424, Serv Informat & Genom, Stn Biol Roscoff, F-29680 Roscoff, France.
5 : CNRS, UMR 7245, Dept RDDM, Museum Natl Hist Nat, CP 52,61 Rue Buffon, F-75231 Paris 05, France.
6 : Univ British Columbia, Dept Zool, 6270 Univ Blvd, Vancouver, BC V6T 1Z4, Canada.
Source European Journal Of Protistology (0932-4739) (Elsevier Gmbh, Urban & Fischer Verlag), 2017-10 , Vol. 61 , N. Part A , P. 265-277
DOI 10.1016/j.ejop.2017.06.007
WOS© Times Cited 10
Keyword(s) Astomatida, Ciliophora, Cirratulidae, Endocytoplasmic bacteria, Microscopy, Molecular phylogeny

Microscopic and phylogenetic analyses were performed on endocommensal astome ciliates retrieved from the middle intestine of a marine cirratulid polychaete, Cirriformia tentaculata, collected in the bay of Roscoff (English Channel, Northwest French coast) and on the Southwest English coast. Three morphotypes of the astome genus Durchoniella were identified, two corresponding to described species (the type species Durchoniella brasili (Léger and Duboscq, 1904) de Puytorac, 1954 and Durchoniella legeriduboscqui de Puytorac, 1954) while a third morphotype remains undescribed. Their small subunit (SSU) rRNA gene sequences showed at least 97.2% identity and phylogenetic analyses grouped them at the base of the subclass Scuticociliatia (Oligohymenophorea), as a sister lineage to all astomes from terrestrial oligochaete annelids. Ultrastructural examination by transmission electron microscopy and fluorescence in situ hybridization analyses revealed the presence of endocytoplasmic cocci and rod-shaped bacteria surrounded by a very thin membrane. These endocytoplasmic bacteria may play a role in the association between endocommensal astome ciliates and cirratulid polychaetes inhabiting in anoxic coastal sediments.

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Sauvadet Anne-Laure, Lynn Denis H., Roussel Erwan, Le Panse Sophie, Bigeard Estelle, Schrevel Joseph, Guillou Laure (2017). Redescription and phylogenetic analyses of Durchoniella spp. (Ciliophora, Astomatida) associated with the polychaete Cirriformia tentaculata (Montagu, 1808). European Journal Of Protistology, 61(Part A), 265-277. Publisher's official version : , Open Access version :