FN Archimer Export Format PT J TI Redescription and phylogenetic analyses of Durchoniella spp. (Ciliophora, Astomatida) associated with the polychaete Cirriformia tentaculata (Montagu, 1808) BT AF SAUVADET, Anne-Laure LYNN, Denis H. ROUSSEL, Erwan LE PANSE, Sophie BIGEARD, Estelle SCHREVEL, Joseph GUILLOU, Laure AS 1:1;2:2,6;3:3;4:4;5:1;6:5;7:1; FF 1:;2:;3:;4:;5:;6:;7:; C1 Univ Pierre & Marie Curie Paris 6, Sorbonne Univ, CNRS, UMR 7144,Stn Biol Roscoff, Pl Georges Teissier,CS90074, F-29688 Roscoff, France. Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. Cardiff Univ, Sch Earth & Ocean Sci, Main Bldg,Pk Pl, Cardiff, S Glam, Wales. CNRS, FR 2424, Serv Informat & Genom, Stn Biol Roscoff, F-29680 Roscoff, France. CNRS, UMR 7245, Dept RDDM, Museum Natl Hist Nat, CP 52,61 Rue Buffon, F-75231 Paris 05, France. Univ British Columbia, Dept Zool, 6270 Univ Blvd, Vancouver, BC V6T 1Z4, Canada. C2 UNIV PARIS 6, FRANCE UNIV GUELPH, CANADA UNIV CARDIFF, UK CNRS, FRANCE MNHN, FRANCE UNIV BRITISH COLUMBIA, CANADA IF 2.43 TC 10 UR https://archimer.ifremer.fr/doc/00392/50312/50990.pdf LA English DT Article DE ;Astomatida;Ciliophora;Cirratulidae;Endocytoplasmic bacteria;Microscopy;Molecular phylogeny AB Microscopic and phylogenetic analyses were performed on endocommensal astome ciliates retrieved from the middle intestine of a marine cirratulid polychaete, Cirriformia tentaculata, collected in the bay of Roscoff (English Channel, Northwest French coast) and on the Southwest English coast. Three morphotypes of the astome genus Durchoniella were identified, two corresponding to described species (the type species Durchoniella brasili (Léger and Duboscq, 1904) de Puytorac, 1954 and Durchoniella legeriduboscqui de Puytorac, 1954) while a third morphotype remains undescribed. Their small subunit (SSU) rRNA gene sequences showed at least 97.2% identity and phylogenetic analyses grouped them at the base of the subclass Scuticociliatia (Oligohymenophorea), as a sister lineage to all astomes from terrestrial oligochaete annelids. Ultrastructural examination by transmission electron microscopy and fluorescence in situ hybridization analyses revealed the presence of endocytoplasmic cocci and rod-shaped bacteria surrounded by a very thin membrane. These endocytoplasmic bacteria may play a role in the association between endocommensal astome ciliates and cirratulid polychaetes inhabiting in anoxic coastal sediments. PY 2017 PD OCT SO European Journal Of Protistology SN 0932-4739 PU Elsevier Gmbh, Urban & Fischer Verlag VL 61 IS Part A UT 000416534000019 BP 265 EP 277 DI 10.1016/j.ejop.2017.06.007 ID 50312 ER EF