FN Archimer Export Format PT J TI Effects of warming rate, acclimation temperature and ontogeny on the critical thermal maximum of temperate marine fish larvae BT AF MOYANO, Marta CANDEBAT, Caroline RUHBAUM, Yannick ALVAREZ-FERNANDEZ, Santiago CLAIREAUX, Guy ZAMBONINO-INFANTE, Jose-Luis PECK, Myron A. AS 1:1;2:1;3:1;4:2;5:3;6:4;7:1; FF 1:;2:;3:;4:;5:;6:PDG-RBE-PFOM-ARN;7:; C1 Univ Hamburg, Inst Hydrobiol & Fisheries Sci, Ctr Earth Syst Res & Sustainabil CEN, Olbersweg 24, Hamburg, Germany. Alfred Wegener Inst Helmholtz Zentrum Polar & Mee, Biol Anstalt Helgoland, Helgoland, Germany. Univ Bretagne Occidentale, Ctr Ifremer Bretagne, LEMAR UMR 6539, Unite PFOM ARN, Plouzane, France. IFREMER, Ctr Ifremer Bretagne, LEMAR UMR 6539, Unite PFOM ARN, Plouzane, France. C2 UNIV HAMBURG, GERMANY INST A WEGENER, GERMANY UBO, FRANCE IFREMER, FRANCE SI BREST SE PDG-RBE-PFOM-ARN UM LEMAR IN WOS Ifremer jusqu'en 2018 DOAJ copubli-france copubli-europe copubli-univ-france IF 2.766 TC 58 UR https://archimer.ifremer.fr/doc/00395/50590/51282.pdf https://archimer.ifremer.fr/doc/00395/50590/51283.pdf https://archimer.ifremer.fr/doc/00395/50590/51284.pdf LA English DT Article AB Most of the thermal tolerance studies on fish have been performed on juveniles and adults, whereas limited information is available for larvae, a stage which may have a particularly narrow range in tolerable temperatures. Moreover, previous studies on thermal limits for marine and freshwater fish larvae (53 studies reviewed here) applied a wide range of methodologies (e.g. the static or dynamic method, different exposure times), making it challenging to compare across taxa. We measured the Critical Thermal Maximum (CTmax) of Atlantic herring (Clupea harengus) and European seabass (Dicentrarchus labrax) larvae using the dynamic method (ramping assay) and assessed the effect of warming rate (0.5 to 9°C h-1) and acclimation temperature. The larvae of herring had a lower CTmax (lowest and highest values among 222 individual larvae, 13.1–27.0°C) than seabass (lowest and highest values among 90 individual larvae, 24.2–34.3°C). At faster rates of warming, larval CTmax significantly increased in herring, whereas no effect was observed in seabass. Higher acclimation temperatures led to higher CTmax in herring larvae (2.7 ± 0.9°C increase) with increases more pronounced at lower warming rates. Pre-trials testing the effects of warming rate are recommended. Our results for these two temperate marine fishes suggest using a warming rate of 3–6°C h-1: CTmax is highest in trials of relatively short duration, as has been suggested for larger fish. Additionally, time-dependent thermal tolerance was observed in herring larvae, where a difference of up to 8°C was observed in the upper thermal limit between a 0.5- or 24-h exposure to temperatures >18°C. The present study constitutes a first step towards a standard protocol for measuring thermal tolerance in larval fish. PY 2017 PD JUN SO Plos One SN 1932-6203 PU Public Library Science VL 12 IS 7 UT 000406575700006 DI 10.1371/journal.pone.0179928 ID 50590 ER EF