TY - JOUR T1 - Determination of stocking density limits for Crassostrea gigas larvae reared in flow-through and recirculating aquaculture systems and interaction between larval density and biofilm formation A1 - Asmani,Katia A1 - Petton,Bruno A1 - Le Grand,Jacqueline A1 - Mounier,Jerome A1 - Robert,Rene A1 - Nicolas,Jean-Louis AD - IFREMER, Ctr Bretagne, UMR LEMAR UBO CNRS IRD Ifremer 6539, Lab Sci Environm Marin, CS 10070, F-29260 Plouzane, France. AD - Univ Brest, Lab Univ Biodiversite & Ecol Microbienne EA3882, ScInBioS IFR148, UEB,ESIAB, Technopole Brest Iroise, F-29280 Plouzane, France. UR - https://archimer.ifremer.fr/doc/00395/50611/ DO - 10.1051/alr/2017023 KW - Pacific oyster KW - Larval culture KW - High stocking density KW - Biofilm KW - Filamentous bacteria N2 - The first aim of this study was to determine the stocking density limits for Pacific oyster Crassostrea gigas larvae reared in flow-through system (FTS) and recirculating aquaculture systems (RAS). The second aim was to examine biofilm formation on the larval tank wall and its interaction with larvae growth. Three larvae concentrations were tested: 50, 150, and 300 mL−1. Chemical parameters and larvae performance were measured. The biofilm was observed by scanning electron microscopy, and its bacterial composition was investigated by pyrosequencing analysis of part of the 16S rRNA gene. The highest growth (13 µm day–1), survival (87%) and metamorphosis (50%) rates were observed in FTS at 50 larvae mL–1, while lower and similar performances occurred at 150 larvae mL–1 in both systems. At 300 larvae mL−1, performances dropped with occurrence of mortality. Biofilm thickness increased with larval density. The pioneer bacteria were coccobacilli followed by filamentous bacteria. The latter constituted abundant braids at the end of rearing at high larval concentrations. The first colonizers were mainly Rhodobacteraceae (α-Proteobacteria). The filamentous bacteria were Saprospirae (Bacteroidetes) and Anaerolineae (Chloroflexi). The biofilm was also made up of other minor groups, including Actinobacteria, Planctomycetes, δ-, γ-Proteobacteria, and Flavobacteriales. The biofilm's composition was more similar to that found in a sewage reactor than in open-sea collectors, which might negatively influence larval rearing due to potential metabolites. This first study on biofilms provides insights into the interaction between rearing density and larvae performance. Y1 - 2017/08 PB - Edp Sciences S A JF - Aquatic Living Resources SN - 0990-7440 VL - 30 IS - 29 SP - 1 EP - 13 ID - 50611 ER -