FN Archimer Export Format
PT J
TI Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)
BT 
AF ZUPA, R.
   FAUVEL, Christian
   MYLONAS, C. C.
   POUSIS, C.
   SANTAMARIA, N.
   PAPADAKI, M.
   FAKRIADIS, I.
   CICIRELLI, V.
   MANGANO, S.
   PASSANTINO, L.
   LACALANDRA, G. M.
   CORRIERO, Aldo
AS 1:1;2:2;3:3;4:1;5:1;6:3;7:3,4;8:1;9:5;10:1;11:1;12:1;
FF 1:;2:PDG-RBE-MARBEC-L3AS;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;
C1 Univ Bari Aldo Moro, Sect Vet Clin & Anim Prod, Dept Emergency & Organ Transplantat, I-70010 Bari, Italy.
   IRD UM2 CNRS IFREMER, Stn Ifremer, UMR MARBEC, F-34250 Palavas Les Flots, France.
   Hellen Ctr Marine Res, Inst Marine Biol Biotechnol & Aquaculture, Iraklion 71003, Crete, Greece.
   Univ Crete, Dept Biol, Iraklion 71003, Crete, Greece.
   CNR, Inst Marine Coastal Environm, I-91021 Capo Granitola, TP, Italy.
C2 UNIV BARI ALDO MORO, ITALY
   IFREMER, FRANCE
   HELLEN CTR MARINE RES, GREECE
   UNIV CRETE, GREECE
   IAMC CNR, ITALY
SI PALAVAS
SE PDG-RBE-MARBEC-L3AS
UM MARBEC
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.711
TC 28
UR https://archimer.ifremer.fr/doc/00395/50613/55414.pdf
LA English
DT Article
DE ;germ cell apoptosis;germ cell proliferation;greater amberjack;rearing in captivity;Seriola dumerili;sperm quality
AB The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of Pou5f1 and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17β-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production. 
PY 2017
PD SEP
SO Journal Of Animal Science
SN 0021-8812
PU Amer Soc Animal Science
VL 95
IS 9
UT 000410813900029
BP 4085
EP 4100
DI 10.2527/jas2017.1708
ID 50613
ER

EF