Response of CO2-starved diatom Phaeodactylum tricornutum to light intensity transition

Type Article
Date 2017-09
Language English
Author(s) Heydarizadeh Parisa1, Boureba Wafaa1, Zahedi Morteza2, Huang Bing1, Moreau Brigitte1, Lukomska Ewa3, Couzinet-Mossion Aurelie4, Wielgosz-Collin Gaetane4, Martin-Jezequel Veronique5, Bougaran GaelORCID3, Marchand Justine1, Schoefs Benoit1
Affiliation(s) 1 : Univ Le Mans, IUML FR CNRS 3473, Mer Mol Sante, Metab Bioengn Microalga Mol & Applicat MIMMA,UBL, F-72085 Le Mans, France.
2 : Isfahan Univ Technol, Dept Agron & Plant Breeding, Coll Agr, Esfahan 8415683111, Iran.
3 : IFREMER, Physiol & Biotechnol Algae Lab, Rue Ile dYeu,BP 21105, F-44311 Nantes, France.
4 : Univ Nantes, Fac Sci Pharmaceut & Biol, Inst Univ Mer & Littoral CNRS FR3473, Grp Mer,Mol,Sante EA 2160, 9 Rue Bias,BP 61112, F-44035 Nantes 1, France.
5 : Univ La Rochelle, UMR CNRS LIENSs 7266, 2 Rue Olympe Gouges, F-17000 La Rochelle, France.
Source Philosophical Transactions Of The Royal Society B-biological Sciences (0962-8436) (Royal Soc), 2017-09 , Vol. 372 , N. 1728 , P. 20160396 (1-9)
DOI 10.1098/rstb.2016.0396
WOS© Times Cited 29
Note Theme issue ‘The peculiar carbon metabolism in diatoms’ compiled and edited by Benoît Schoefs, Hanhua Hu and Peter G. Kroth Electronic supplementary material is available online at https://dx.doi.org/10.6084/m9.figshare.c.3800485.
Keyword(s) Phaeodactylum tricornutum, carbon metabolism, light regulation, photosynthesis, chlorophyll fluorescence, pyruvate hub
Abstract

In this study, we investigated the responses of Phaeodactylum tricornutum cells acclimated to 300 mu mol m(-2) s(-1) photon flux density to an increase (1000 mmol m(-2) s(-1)) or decrease (30 mmol m(-2)s(-1)) in photon flux densities. The light shift occurred abruptly after 5 days of growth and the acclimation to new conditions was followed during the next 6 days at the physiological and molecular levels. The molecular data reflect a rearrangement of carbon metabolism towards the production of phosphoenolpyruvic acid (PEP) and/or pyruvate. These intermediates were used differently by the cell as a function of the photon flux density: under low light, photosynthesis was depressed while respiration was increased. Under high light, lipids and proteins accumulated. Of great interest, under high light, the genes coding for the synthesis of aromatic amino acids and phenolic compounds were upregulated suggesting that the shikimate pathway was activated.

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Heydarizadeh Parisa, Boureba Wafaa, Zahedi Morteza, Huang Bing, Moreau Brigitte, Lukomska Ewa, Couzinet-Mossion Aurelie, Wielgosz-Collin Gaetane, Martin-Jezequel Veronique, Bougaran Gael, Marchand Justine, Schoefs Benoit (2017). Response of CO2-starved diatom Phaeodactylum tricornutum to light intensity transition. Philosophical Transactions Of The Royal Society B-biological Sciences, 372(1728), 20160396 (1-9). Publisher's official version : https://doi.org/10.1098/rstb.2016.0396 , Open Access version : https://archimer.ifremer.fr/doc/00395/50620/