FN Archimer Export Format PT J TI Response of CO2-starved diatom Phaeodactylum tricornutum to light intensity transition BT AF HEYDARIZADEH, Parisa BOUREBA, Wafaa ZAHEDI, Morteza HUANG, Bing MOREAU, Brigitte LUKOMSKA, Ewa COUZINET-MOSSION, Aurelie WIELGOSZ-COLLIN, Gaetane MARTIN-JEZEQUEL, Veronique BOUGARAN, Gael MARCHAND, Justine SCHOEFS, Benoit AS 1:1;2:1;3:2;4:1;5:1;6:3;7:4;8:4;9:5;10:3;11:1;12:1; FF 1:;2:;3:;4:;5:;6:PDG-RBE-BRM-PBA;7:;8:;9:;10:PDG-RBE-BRM-PBA;11:;12:; C1 Univ Le Mans, IUML FR CNRS 3473, Mer Mol Sante, Metab Bioengn Microalga Mol & Applicat MIMMA,UBL, F-72085 Le Mans, France. Isfahan Univ Technol, Dept Agron & Plant Breeding, Coll Agr, Esfahan 8415683111, Iran. IFREMER, Physiol & Biotechnol Algae Lab, Rue Ile dYeu,BP 21105, F-44311 Nantes, France. Univ Nantes, Fac Sci Pharmaceut & Biol, Inst Univ Mer & Littoral CNRS FR3473, Grp Mer,Mol,Sante EA 2160, 9 Rue Bias,BP 61112, F-44035 Nantes 1, France. Univ La Rochelle, UMR CNRS LIENSs 7266, 2 Rue Olympe Gouges, F-17000 La Rochelle, France. C2 UNIV LE MANS, FRANCE UNIV ESFAHAN, IRAN IFREMER, FRANCE UNIV NANTES, FRANCE UNIV LA ROCHELLE, FRANCE SI NANTES SE PDG-RBE-BRM-PBA IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france copubli-int-hors-europe copubli-sud IF 5.666 TC 41 UR https://archimer.ifremer.fr/doc/00395/50620/52341.pdf LA English DT Article DE ;Phaeodactylum tricornutum;carbon metabolism;light regulation;photosynthesis;chlorophyll fluorescence;pyruvate hub AB In this study, we investigated the responses of Phaeodactylum tricornutum cells acclimated to 300 mu mol m(-2) s(-1) photon flux density to an increase (1000 mmol m(-2) s(-1)) or decrease (30 mmol m(-2)s(-1)) in photon flux densities. The light shift occurred abruptly after 5 days of growth and the acclimation to new conditions was followed during the next 6 days at the physiological and molecular levels. The molecular data reflect a rearrangement of carbon metabolism towards the production of phosphoenolpyruvic acid (PEP) and/or pyruvate. These intermediates were used differently by the cell as a function of the photon flux density: under low light, photosynthesis was depressed while respiration was increased. Under high light, lipids and proteins accumulated. Of great interest, under high light, the genes coding for the synthesis of aromatic amino acids and phenolic compounds were upregulated suggesting that the shikimate pathway was activated. PY 2017 PD SEP SO Philosophical Transactions Of The Royal Society B-biological Sciences SN 0962-8436 PU Royal Soc VL 372 IS 1728 UT 000405676200001 DI 10.1098/rstb.2016.0396 ID 50620 ER EF