FN Archimer Export Format
PT J
TI Purification of the exopolysaccharide produced by Alteromonas infernus: identification of endotoxins and effective process to remove them
BT 
AF GRIVAUD-LE DU, Alicia
   ZYKWINSKA, Agata
   SINQUIN, Corinne
   RATISKOL, Jacqueline
   WEISS, Pierre
   VINATIER, Claire
   GUICHEUX, Jerome
   DELBARRE-LADRAT, Christine
   COLLIEC-JOUAULT, Sylvia
AS 1:1;2:1;3:1;4:1;5:2;6:2;7:2;8:1;9:1;
FF 1:PDG-RBE-BRM-LEMMMB;2:PDG-RBE-BRM-LEMMMB;3:PDG-RBE-BRM-LEMMMB;4:PDG-RBE-BRM-LEMMMB;5:;6:;7:;8:PDG-RBE-BRM-LEMMMB;9:PDG-RBE-BRM-LEMMMB;
C1 IFREMER, Lab Ecosyst Microbiens & Mol Marines Biotechnol, EM3B, Rue Ile dYeu, F-44311 Nantes 3, France.
   Univ Nantes, Inserm UMRS 1229, UFR Odontol, 1 Pl Alexis Ricordeau, F-44042 Nantes 1, France.
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.34
TC 4
UR https://archimer.ifremer.fr/doc/00397/50818/51601.pdf
LA English
DT Article
DE ;Marine Alteromonas infernus;Bacterial exopolysaccharide;Endotoxin;Characterization;Purification;Fermentation;Process
AB Alteromonas infernus bacterium isolated from deep-sea hydrothermal vents can produce by fermentation a high molecular weight exopolysaccharide (EPS) called GY785. This EPS described as a new source of glycosaminoglycan-like molecule presents a great potential for pharmaceutical and biotechnological applications. However, this unusual EPS is secreted by a Gram-negative bacterium and can be therefore contaminated by endotoxins, in particular the lipopolysaccharides (LPS). Biochemical and chemical analyses of the LPS extracted from A. infernus membranes have shown the lack of the typical LPS architecture since 3-deoxy-d-manno-oct-2-ulopyranosonic acid (Kdo), glucosamine (GlcN), and phosphorylated monosaccharides were not present. Unlike for other Gram-negative bacteria, the results revealed that the outer membrane of A. infernus bacterium is most likely composed of peculiar glycolipids. Furthermore, the presence of these glycolipids was also detected in the EPS batches produced by fermentation. Different purification and chemical detoxification methods were evaluated to efficiently purify the EPS. Only the method based on a differential solubility of EPS and glycolipids in deoxycholate detergent showed the highest decrease in the endotoxin content. In contrast to the other tested methods, this new protocol can provide an effective method for obtaining endotoxin-free EPS without any important modification of its molecular weight, monosaccharide composition, and sulfate content. 
PY 2017
PD SEP
SO Applied Microbiology And Biotechnology
SN 0175-7598
PU Springer
VL 101
IS 17
UT 000407563000003
BP 6597
EP 6606
DI 10.1007/s00253-017-8364-8
ID 50818
ER

EF