First record of resting cysts of the benthic dinoflagellate Prorocentrum leve in a natural reservoir in Gujan-Mestras, Gironde, France

Type Article
Date 2017-12
Language English
Author(s) Mertens KennethORCID1, Gu Haifeng2, Pospelova Vera3, Chomerat Nicolas1, Nezan Elisabeth1, Gurdebeke Pieter4, Bogus Kara5, Vrielinck Henk6, Rumebe Myriam7, Meteigner Claire7
Affiliation(s) 1 : IFREMER, Stn Biol Marine, LER BO, Pl Croix,BP40537, F-29185 Concarneau, France.
2 : SOA, Inst Oceanog 3, Xiamen 361005, Peoples R China.
3 : Univ Victoria, Sch Earth & Ocean Sci, STN CSC, POB 1700, Victoria, BC V8W 2Y2, Canada.
4 : Univ Ghent, Dept Geol, Krijgslaan 281,S8, B-9000 Ghent, Belgium.
5 : Texas A&M Univ, Int Ocean Discovery Program, College Stn, TX 77845 USA.
6 : Univ Ghent, Dept Solid State Sci, Krijgslaan 281,S1, B-9000 Ghent, Belgium.
7 : IFREMER, Stn Arcachon, LER AR, F-33120 Arcachon, France.
Source Journal Of Phycology (0022-3646) (Wiley), 2017-12 , Vol. 53 , N. 6 , P. 1193-1205
DOI 10.1111/jpy.12582
WOS© Times Cited 7
Keyword(s) cellulosic, micro-FTIR, palynology, Prorocentrales, resting stage
Abstract

The resting cysts of the benthic dinoflagellate Prorocentrum leve from a natural reservoir in Gujan-Mestras (Gironde, France) were described in this study. The incubated urn-shaped cysts gave rise to cells of Prorocentrum leve. Morphological observations through light microscopy and scanning electron microscopy, particularly of the periflagellar platelets, combined with large subunit ribosomal DNA sequences obtained through single-cell analysis confirm their affinity to the species Prorocentrum leve. The cysts are characterized by a specific shape and the presence of an anterior plug. This is the first conclusive evidence for fossilizable resting stages within the Prorocentrales, one of the major orders within the Dinophyceae. Palynological treatments show that the cysts and endospores withstand hydrochloric and hydrofluoric acids. MicroFTIR analysis on single specimens suggests that the composition of the endospore is cellulosic and the cyst wall a more robust, non-cellulosic β-glucan. The spectra overall are similar to other published spectra of resting cysts from autotrophic, planktonic dinoflagellates.

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Mertens Kenneth, Gu Haifeng, Pospelova Vera, Chomerat Nicolas, Nezan Elisabeth, Gurdebeke Pieter, Bogus Kara, Vrielinck Henk, Rumebe Myriam, Meteigner Claire (2017). First record of resting cysts of the benthic dinoflagellate Prorocentrum leve in a natural reservoir in Gujan-Mestras, Gironde, France. Journal Of Phycology, 53(6), 1193-1205. Publisher's official version : https://doi.org/10.1111/jpy.12582 , Open Access version : https://archimer.ifremer.fr/doc/00398/50971/