FN Archimer Export Format
PT J
TI Haemocytes from Crassostrea gigas and OsHV-1: A promising in vitro system to study host/virus interactions
BT 
AF MORGA, Benjamin
   FAURY, Nicole
   GUESDON, Stephane
   CHOLLET, Bruno
   RENAULT, Tristan
AS 1:1;2:1;3:2;4:1;5:3;
FF 1:PDG-RBE-SG2M-LGPMM;2:PDG-RBE-SG2M-LGPMM;3:PDG-ODE-LITTORAL-LERPC;4:PDG-RBE-SG2M-LGPMM;5:PDG-RBE;
C1 IFREMER, Lab Genet & Pathol Mollusques Marins, La Tremblade, France.
   IFREMER, LER PC, La Tremblade, France.
   IFREMER, Dept Ressources Biol & Environm, Nantes, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   NANTES
SE PDG-RBE-SG2M-LGPMM
   PDG-ODE-LITTORAL-LERPC
   PDG-RBE
IN WOS Ifremer jusqu'en 2018
IF 2.511
TC 21
UR https://archimer.ifremer.fr/doc/00399/50997/51780.pdf
LA English
DT Article
AB Since 2008, mass mortality outbreaks associated with the detection of particular variants of OsHV-1 have been reported in Crassostrea gigas spat and juveniles in several countries. Recent studies have reported information on viral replication during experimental infection. Viral DNA and RNA were also detected in the haemolymph and haemocytes suggesting that the virus could circulate through the circulatory system. However, it is unknown if the virus is free in the haemolymph, passively associated at the surface of haemocytes, or able to infect and replicate inside these cells inducing (or not) virion production. In the present study, we collected haemocytes from the haemolymphatic sinus of the adductor muscle of healthy C. gigas spat and exposed them in vitro to a viral suspension. Results showed that viral RNAs were detectable one hour after contact and the number of virus transcripts increased over time in association with an increase of viral DNA detection. These results suggested that the virus is able to initiate replication rapidly inside haemocytes maintained in vitro. These in vitro trials were also used to carry out a dual transcriptomic study. We analyzed concomitantly the expression of some host immune genes and 15 viral genes. Results showed an up regulation of oyster genes currently studied during OsHV-1 infection. Additionally, transmission electron microscopy examination was carried out and did not allow the detection of viral particles. Moreover, All the results suggested that the in vitro model using haemocytes can be valuable for providing new perspective on virus-oyster interactions. 
PY 2017
PD NOV
SO Journal Of Invertebrate Pathology
SN 0022-2011
PU Academic Press Inc Elsevier Science
VL 150
UT 000416298200008
BP 45
EP 53
DI 10.1016/j.jip.2017.09.007
ID 50997
ER

EF