FN Archimer Export Format
PT J
TI Early exposure to chronic hypoxia induces short- and long-term regulation of hemoglobin gene expression in European sea bass (Dicentrarchus labrax)
BT 
AF CADIZ, Laura
   SERVILI, Arianna
   QUAZUGUEL, Patrick
   MADEC, Lauriane
   ZAMBONINO-INFANTE, Jose-Luis
   MAZURAIS, David
AS 1:1;2:1;3:1;4:1;5:1;6:1;
FF 1:;2:PDG-RBE-PFOM-ARN;3:PDG-RBE-PFOM-ARN;4:PDG-RBE-PFOM-ARN;5:PDG-RBE-PFOM-ARN;6:PDG-RBE-PFOM-ARN;
C1 IFREMER, Ctr Bretagne, Unite Physiol Fonct Organismes Marins, LEMAR,UMR 6539, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
SI BREST
SE PDG-RBE-PFOM-ARN
UM LEMAR
IN WOS Ifremer jusqu'en 2018
IF 3.179
TC 17
UR https://archimer.ifremer.fr/doc/00399/51039/51831.pdf
LA English
DT Article
DE ;Hypoxia;Hemoglobin;Early exposure;European sea bass
AB European sea bass (Dicentrarchus labrax) inhabits coastal waters and may be exposed to hypoxia at different life stages, requiring physiological and behavioral adaptation. In the present study, we attempted to determine whether regulation of hemoglobin (Hb) gene expression plays a role in the physiological response to chronic moderate hypoxia in whole larvae and hematopoietic tissues (head kidney and spleen) of juveniles. We also tested the hypothesis that hypoxia exposure at the larval stage could induce a long-term effect on the regulation of Hb gene expression. For this purpose, D. labrax were exposed to a non-lethal hypoxic condition (40% air saturation) at the larval stage from 28 to 50 days post-hatching (dph) and/or at the juvenile stage from 196 to 296 dph. Data obtained from larvae indicate that hypoxia induced a subtype-specific regulation of Hb gene expression, with a significant decrease of MN-Hba3, MN-Hb beta 4 and MN-Hb beta 5 and increase of MN-Hb alpha 2, LA-Hb alpha 1 and LA-Hb beta 1 transcript levels. Hypoxia did not induce regulation of Hb gene expression in juveniles, except in the head kidney for those that experienced hypoxia at the larval stage. The latter exhibited a significant hypoxia-induced stimulation of MN-Hba2, LA-Hba1 and LA-Hb beta 1 gene expression, associated with stimulation of the PHD-3 gene involved in the hypoxia-inducible factor oxygen-sensing pathway. We conclude that subtype-and stage-specific regulation of Hb gene expression plays a role in the physiological response of D. labrax to cope with hypoxia and that early exposure to low oxygen concentration has a long-term effect on this response. 
PY 2017
PD SEP
SO Journal Of Experimental Biology
SN 0022-0949
PU Company Of Biologists Ltd
VL 220
IS 17
UT 000408631800021
BP 3119
EP 3126
DI 10.1242/jeb.160713
ID 51039
ER

EF