Infection dynamics of a V. splendidus strain pathogenic to Mytilus edulis : In vivo and in vitro interactions with hemocytes

Type Article
Date 2017-11
Language English
Author(s) Ben Cheikh YosraORCID1, Travers Marie-AgnesORCID2, Le Foll Frank1
Affiliation(s) 1 : Univ Le Havre Normandie, FR CNRS Scale 3730, Environm Stresses & Aquat Biomonitoring, UMR I INERIS URCA ULH SEBIO 02, F-76063 Le Havre, France.
2 : IFREMER, SG2M, LGPMM, Ave Mus de Loup, F-17390 La Tremblade, France.
Source Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2017-11 , Vol. 70 , P. 515-523
DOI 10.1016/j.fsi.2017.09.047
WOS© Times Cited 8
Keyword(s) Innate immunity, Bivalves, Vibrio, Infection, Experimental model, Hemocyte

The pathogenic strain V. splendidus 10/068 1T1 has previously been reported for its virulence to the blue mussel and for its capacity to alter immune responses. In this study, we expanded the knowledge on hemocyte-pathogen interactions by using in vitro and in vivo assays. V. splendidus 10/068 1T1 severely inhibited cell adhesion and acidic vacuole formation unlike the innocuous phylogenetically related V. splendidus 12/056 M24T1 which had no effect on these cell functions. Furthermore, the virulent bacteria decreased hemocyte viability (59% of viability after 24 h). Infection dynamics were explored by using a model based on water tank cohabitation with septic mussels infected by GFP-tagged V. splendidus 10/068 1T1. Experimental infections were successfully produced (16.6% and 45% mortalities in 3 days and 6 days). The amount of GFP Vibrio in seawater decreased during the experiment suggesting its horizontal transfer from diseased animals to healthy ones. At the same time periods, bacteria were detected in hemocytes and in various organs and caused necrosis especially in gills. Total hemocyte count and viability were affected. Taken together, our results indicate that the pathogen V. splendidus 10/068 1T1 colonizes its host both by bypassing external defense barriers and impairing hemocyte defense activities.

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