Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production

Type Article
Date 2017-09
Language English
Author(s) Ben Amor Faten1, 2, Elleuch Fatma2, 3, Ben Hlima Hajer1, Garnier MatthieuORCID4, Saint-Jean BrunoORCID4, Barkallah Mohamed1, Pichon Chantal3, Abdelkafi Slim1, Fendri Imen2
Affiliation(s) 1 : Univ Sfax, Natl Sch Engineers Sfax, Biol Engn Dept, Unite Biotechnol Algues, Sfax 3038, Tunisia.
2 : Univ Sfax, Fac Sci Sfax, Lab Biotechnol Vegetale Appl Ameliorat Cultures, Sfax 3000, Tunisia.
3 : CNRS UPR 4301, Ctr Mol Biophys CBM, F-45071 Orleans, France.
4 : IFREMER, Lab BRM PBA, F-44311 Nantes, France.
Source Marine Drugs (1660-3397) (Mdpi Ag), 2017-09 , Vol. 15 , N. 9 , P. 293 (1-16)
DOI 10.3390/md15090293
WOS© Times Cited 14
Keyword(s) proteome, Dunaliella, 18S rRNA gene, carotenoids, bidimensional gel electrophoresis, mass spectrometry

The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel electrophoresis, was performed from standard and optimized cultures. We detected 127 protein spots which were significantly differentially expressed between standard and optimized cultures. Among them 16 protein spots were identified with mass spectrometry and grouped into different functional categories using KEGG (Kyoto Encyclopedia of Genes and Genomes) such as photosynthetic Calvin cycle, regulation/defense, energy metabolism, glycolysis, and cellular processes. The current study could be of great interest in providing information on the effect of stressful conditions in microalgae carotenoid production.

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Ben Amor Faten, Elleuch Fatma, Ben Hlima Hajer, Garnier Matthieu, Saint-Jean Bruno, Barkallah Mohamed, Pichon Chantal, Abdelkafi Slim, Fendri Imen (2017). Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production. Marine Drugs, 15(9), 293 (1-16). Publisher's official version : , Open Access version :