FN Archimer Export Format
PT J
TI Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
BT 
AF BEN AMOR, Faten
   ELLEUCH, Fatma
   BEN HLIMA, Hajer
   GARNIER, Matthieu
   SAINT-JEAN, Bruno
   BARKALLAH, Mohamed
   PICHON, Chantal
   ABDELKAFI, Slim
   FENDRI, Imen
AS 1:1,2;2:2,3;3:1;4:4;5:4;6:1;7:3;8:1;9:2;
FF 1:;2:;3:;4:PDG-RBE-BRM-PBA;5:PDG-RBE-BRM-PBA;6:;7:;8:;9:;
C1 Univ Sfax, Natl Sch Engineers Sfax, Biol Engn Dept, Unite Biotechnol Algues, Sfax 3038, Tunisia.
   Univ Sfax, Fac Sci Sfax, Lab Biotechnol Vegetale Appl Ameliorat Cultures, Sfax 3000, Tunisia.
   CNRS UPR 4301, Ctr Mol Biophys CBM, F-45071 Orleans, France.
   IFREMER, Lab BRM PBA, F-44311 Nantes, France.
C2 UNIV SFAX, TUNISIA
   UNIV SFAX, TUNISIA
   CNRS, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-RBE-BRM-PBA
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-int-hors-europe
   copubli-sud
IF 4.379
TC 17
UR https://archimer.ifremer.fr/doc/00402/51398/51973.pdf
LA English
DT Article
DE ;proteome;Dunaliella;18S rRNA gene;carotenoids;bidimensional gel electrophoresis;mass spectrometry
AB The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel electrophoresis, was performed from standard and optimized cultures. We detected 127 protein spots which were significantly differentially expressed between standard and optimized cultures. Among them 16 protein spots were identified with mass spectrometry and grouped into different functional categories using KEGG (Kyoto Encyclopedia of Genes and Genomes) such as photosynthetic Calvin cycle, regulation/defense, energy metabolism, glycolysis, and cellular processes. The current study could be of great interest in providing information on the effect of stressful conditions in microalgae carotenoid production. 
PY 2017
PD SEP
SO Marine Drugs
SN 1660-3397
PU Mdpi Ag
VL 15
IS 9
UT 000411559800032
DI 10.3390/md15090293
ID 51398
ER

EF