FN Archimer Export Format
PT J
TI Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae
BT 
AF MORGA, Benjamin
   RENAULT, Tristan
   FAURY, Nicole
   LEROND, Sophie
   GARCIA, Celine
   CHOLLET, Bruno
   JOLY, Jean-Pierre
   LAPEGUE, Sylvie
   HARRANG, Estelle
   ARZUL, Isabelle
AS 1:1;2:2;3:1;4:1;5:1;6:1;7:1;8:1;9:1;10:1;
FF 1:PDG-RBE-SG2M-LGPMM;2:PDG-RBE;3:PDG-RBE-SG2M-LGPMM;4:;5:PDG-RBE-SG2M-LGPMM;6:PDG-RBE-SG2M-LGPMM;7:PDG-RBE-SG2M-LGPMM;8:PDG-RBE-SG2M;9:PDG-RBE-SG2M-LGPMM;10:PDG-RBE-SG2M-LGPMM;
C1 Inst Francais Rech Exploitat Mer, Lab Genet & Pathol Mollusques Marins, IFREMER, La Tremblade, France.
   Inst Francais Rech Exploitat Mer, Dept Ressources Biol & Environm, IFREMER, Nantes, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   NANTES
SE PDG-RBE-SG2M-LGPMM
   PDG-RBE
   PDG-RBE-SG2M
IN WOS Ifremer jusqu'en 2018
   DOAJ
IF 3.52
TC 11
UR https://archimer.ifremer.fr/doc/00405/51665/52213.pdf
LA English
DT Article
DE ;Bonamia ostreae;protozoan;Ostrea edulis;resistance;experimental infection;haemocytes;gene expression;apoptosis
AB Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and phagocytosis activity were analyzed by flow cytometry. Four genes homologous to those shown to be involved in immunity were selected (Inhibitor of apotosis OeIAP, Fas ligand OeFas-ligand, Oe-SOD, and OeEc-SOD) and monitored by quantitative reverse-transcription PCR (qRT-PCR). Infected oysters showed higher phagocytosis activity than controls. Infected selected oyster show a lower phagocytosis activity which might be a protection against the parasite infection. The expression of OeIAP and OeFas-ligand gene was significantly increased in selected oysters at 5 days post-injection. OeIAP gene expression appeared to be significantly increased in wild-type oysters at 8 days post-injection. Our results suggest that resistance to bonamiosis partly relies on the ability of the oysters to modulate apoptosis. 
PY 2017
PD OCT
SO Frontiers In Cellular And Infection Microbiology
SN 2235-2988
PU Frontiers Media Sa
VL 7
IS 433
UT 000412503500001
BP 1
EP 13
DI 10.3389/fcimb.2017.00433
ID 51665
ER

EF