FN Archimer Export Format
PT J
TI An immune-related gene expression atlas of the shrimp digestive system in response to two major pathogens brings insights into the involvement of hemocytes in gut immunity
BT 
AF SILVEIRA, Amanda S.
   MATOS, Gabriel M.
   FALCHETTI, Marcelo
   RIBEIRO, Fabio S.
   BRESSAN, Albert
   BACHERE, Evelyne
   PERAZZOLO, Luciane M.
   ROSA, Rafael D.
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:1;8:1;
FF 1:;2:;3:;4:;5:;6:PDG-RBE-IHPE;7:;8:;
C1 Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, Lab Immunol Appl Aquaculture, BR-88040900 Florianopolis, SC, Brazil.
   Univ Montpellier, CNRS, UPVD, Ifremer,UMR 5244,IHPE, CC 080, F-34095 Montpellier, France.
C2 UNIV FED SANTA CATARINA, BRAZIL
   IFREMER, FRANCE
SI MONTPELLIER
SE PDG-RBE-IHPE
UM IHPE
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
   copubli-sud
IF 3.119
TC 32
UR https://archimer.ifremer.fr/doc/00406/51735/52304.pdf
   https://archimer.ifremer.fr/doc/00406/51735/52305.pdf
   https://archimer.ifremer.fr/doc/00406/51735/52306.pdf
LA English
DT Article
DE ;Crustacean;Litopenaeus vannamei;Intestinal immunity;Hemocyte;Antimicrobial peptide;Anti-lipopolysaccharide factor
AB Much of our current knowledge on shrimp immune system is restricted to the defense reactions mediated by the hemocytes and little is known about gut immunity. Here, we have investigated the transcriptional profile of immune-related genes in different organs of the digestive system of the shrimp Litopenaeus vannamei. First, the tissue distribution of 52 well-known immune-related genes has been assessed by semiquantitative analysis in the gastrointestinal tract (foregut, midgut and hindgut) and in the hepatopancreas and circulating hemocytes of shrimp stimulated or not with heat-killed bacteria. Then, the expression levels of 18 genes from key immune functional categories were quantified by fluorescence-based quantitative PCR in the midgut of animals experimentally infected with the Gram-negative Vibrio harveyi or the White spot syndrome virus (WSSV). Whereas the expression of some genes was induced at 48 h after the bacterial infection, any of the analyzed genes showed to be modulated in response to the virus. Whole-mount immunofluorescence assays confirmed the presence of infiltrating hemocytes in the intestines, indicating that the expression of some immune-related genes in gut is probably due to the migratory behavior of these circulating cells. This evidence suggests the participation of hemocytes in the delivery of antimicrobial molecules into different portions of the digestive system. Taken all together, our results revealed that gut is an important immune organ in L. vannamei with intimate association with hemocytes. 
PY 2018
PD FEB
SO Developmental And Comparative Immunology
SN 0145-305X
PU Elsevier Sci Ltd
VL 79
UT 000422811500005
BP 44
EP 50
DI 10.1016/j.dci.2017.10.005
ID 51735
ER

EF