FN Archimer Export Format PT J TI Effect of vacuum and modified atmosphere packaging on the microbiological, chemical and sensory properties of tropical red drum ( Sciaenops ocellatus ) fillets stored at 4 °C BT AF SILBANDE, Adele ADENET, Sandra CHOPIN, Christine CORNET, Josiane SMITH-RAVIN, Juliette ROCHEFORT, Katia LEROI, Francoise AS 1:1,2,3;2:1;3:2;4:2;5:3;6:1;7:2; FF 1:;2:;3:PDG-RBE-BRM-LEMMMB;4:PDG-RBE-BRM-LEMMMB;5:;6:;7:PDG-RBE-BRM-LEMMMB; C1 PARM, Impasse Petit Morne 375, F-97232 Lamentin, Martinique, France. IFREMER, Lab Ecosyst Microbiens & Mol Marines Biotechnol, EM3B, Rue Tle Yeu,BP 21105, F-44300 Nantes, France. Univ Antilles, Dept Sci Inter Fac, DSI, AIHP EA929,GEODE,Grp BIOSPHERES, BP 7209, F-97275 Schoelcher, Martinique, France. C2 PARM, FRANCE IFREMER, FRANCE UNIV ANTILLES, FRANCE SI NANTES SE PDG-RBE-BRM-LEMMMB IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 4.006 TC 43 UR https://archimer.ifremer.fr/doc/00407/51858/52468.pdf LA English DT Article DE ;Seafood;Biogenic amine;VP;MAP;NGS;16S rRNA gene AB Aims The effect of vacuum (VP – 4 °C) and CO2/N2–atmosphere (MAP – 4 °C) packaging on the quality of red drum fillets compared with whole gutted iced fish was investigated. Methods and results A metagenomic approach, bacterial enumeration and isolation, biochemical and sensory analyses were carried out. The organoleptic rejection of whole fish was observed at day 15 whereas VP and MAP fillets appeared unacceptable only after 29 days. At these dates, total mesophilic counts reached 107–108 CFU g− 1. According to Illumina MiSeq sequencing, Arthrobacter, Chryseobacterium, Brevibacterium, Staphylococcus and Kocuria were the main genera of the fresh red drum fillets. At the sensory rejection time, lactic acid bacteria (LAB), particularly Carnobacterium sp., dominated the microbiota of both types of packaging. The pH value of fresh samples was between 5.96 and 6.37 and did not vary greatly in all trials. Total volatile basic nitrogen (TVBN) and trimethylamine (TMA) concentrations were low and not represent reliable indicators of the spoilage, contrary to some biogenic amines (cadaverine, putrescine and tyramine). Conclusion Chilled packed fillets of red drum have an extended shelf-life compared to whole gutted iced fish. Overall, few differences in sensory and microbial quality were observed between the VP and MAP samples. Significance and impact of the study Next-Generation Sequencing (NGS) provided data on the microbiota of a tropical fish. PY 2018 PD FEB SO International Journal Of Food Microbiology SN 0168-1605 PU Elsevier Science Bv VL 266 UT 000424185900005 BP 31 EP 41 DI 10.1016/j.ijfoodmicro.2017.10.015 ID 51858 ER EF