FN Archimer Export Format
PT J
TI Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication
BT 
AF PAULETTO, Marianna
   SEGARRA, Amelie
   MONTAGNANI, Caroline
   QUILLIEN, Virgile
   FAURY, Nicole
   LE GRAND, Jacqueline
   MINER, Philippe
   PETTON, Bruno
   LABREUCHE, Yannick
   FLEURY, Elodie
   FABIOUX, Caroline
   BARGELLONI, Luca
   RENAULT, Tristan
   HUVET, Arnaud
AS 1:1;2:2;3:3;4:4;5:2;6:4;7:4;8:4;9:5;10:4;11:6;12:1;13:7;14:4;
FF 1:;2:;3:PDG-RBE-IHPE;4:PDG-RBE-PFOM-LPI;5:PDG-RBE-SG2M-LGPMM;6:PDG-RBE-PFOM-PI;7:PDG-RBE-PFOM-PI;8:PDG-RBE-PFOM-PI;9:PDG-RBE-PFOM;10:PDG-RBE-PFOM-PI;11:;12:;13:PDG-RBE;14:PDG-RBE-PFOM-PI;
C1 Univ Padua, Dept Comparat Biomed & Food Sci, Viale Univ 16, I-35020 Legnaro, PD, Italy.
   IFREMER, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France.
   Univ Montpellier, IFREMER, Univ Perpignan Via Domitia, IHPE UMR 5244,CNRS, F-34095 Montpellier, France.
   UBO, UMR CNRS 6539, IFREMER, IRD,LEMAR, F-29280 Plouzane, France.
   UPMC Paris 06, Sorbonne Univ, CNRS, UMR 8227,Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France.
   Univ Bretagne Occidentale, UMR CNRS 6539, IFREMER, Inst Univ Europeen Mer,IRD,LEMAR, F-29280 Plouzane, France.
   IFREMER, Dept Ressources Biol & Environm, Rue lIle dYeu, F-44000 Nantes, France.
C2 UNIV PADUA, ITALY
   IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
   UBO, FRANCE
   UNIV PARIS 06, FRANCE
   UBO, FRANCE
   IFREMER, FRANCE
SI MONTPELLIER
   BREST
   LA TREMBLADE
   ARGENTON
   ROSCOFF
   NANTES
SE PDG-RBE-IHPE
   PDG-RBE-PFOM-LPI
   PDG-RBE-SG2M-LGPMM
   PDG-RBE-PFOM-PI
   PDG-RBE-PFOM
   PDG-RBE
UM LEMAR
   IHPE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3.179
TC 13
UR https://archimer.ifremer.fr/doc/00409/52011/52698.pdf
LA English
DT Article
DE ;Anti-viral response;RNA interference;Inhibitor of NF-kappa B;Marine bivalve;Ostreid herpesvirus 1
AB Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters before being challenged by OsHV-1. Survival appeared close to 100% in both dsRNA-injected conditions associated with a low detection of viral DNA and a low expression of a panel of 39 OsHV-1 genes as compared with infected control. Long dsRNA molecules, both Cg-I kappa B2- and GFP-dsRNA, may have induced an anti-viral state controlling the OsHV-1 replication and precluding the understanding of the specific role of Cg-I kappa B2. Immune-related genes including Cg-I kappa B1, Cg-Rel1, Cg-IFI44, Cg-PKR and Cg-IAP appeared activated in the dsRNA-injected condition, potentially hampering viral replication and thus conferring a better resistance to OsHV-1 infection. We revealed that long dsRNA-mediated genetic interference triggered an anti-viral state in the oyster, emphasizing the need for new reverse genetics tools for assessing immune gene function and avoiding off-target effects in bivalves. 
PY 2017
PD OCT
SO Journal Of Experimental Biology
SN 0022-0949
PU Company Of Biologists Ltd
VL 220
IS 20
UT 000413196900013
BP 3671
EP 3685
DI 10.1242/jeb.156299
ID 52011
ER

EF