Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel ( Anguilla anguilla ) larvae

Type Article
Date 2018-04
Language English
Author(s) Politis Sebastian Nikitas1, Servili Arianna2, Mazurais DavidORCID2, Zambonino-Infante Jose-LuisORCID2, Miest J. J.3, 4, Tomkiewicz J.1, Butts I. A. E.1, 5
Affiliation(s) 1 : Tech Univ Denmark, Natl Inst Aquat Resources, Lyngby, Denmark.
2 : IFREMER, Marine Environm Sci Lab, UMR 6539, Plouzane, France.
3 : Helmholtz Ctr Ocean Res, Kiel, Germany.
4 : Univ Greenwich, Dept Life & Sports Sci, London, Kent, England.
5 : Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA.
Source General And Comparative Endocrinology (0016-6480) (Academic Press Inc Elsevier Science), 2018-04 , Vol. 259 , P. 54-65
DOI 10.1016/j.ygcen.2017.11.003
WOS© Times Cited 12
Keyword(s) Aquaculture, Fish, Early life history
Abstract

Thyroid hormones (THs) are key regulators of growth, development, and metabolism in vertebrates and influence early life development of fish. TH is produced in the thyroid gland (or thyroid follicles) mainly as T4 (thyroxine), which is metabolized to T3 (3,5,3’-triiodothyronine) and T2 (3,5-diiodothyronine) by deiodinase (DIO) enzymes in peripheral tissues. The action of these hormones is mostly exerted by binding to a specific nuclear thyroid hormone receptor (THR). In this study, we i) cloned and characterized thr sequences, ii) investigated the expression pattern of the different subtypes of thrs and dios, and iii) studied how temperature affects the expression of those genes in artificially produced early life history stages of European eel (Anguilla anguilla), reared in different thermal regimes (16, 18, 20 and 22°C) from hatch until first-feeding. We identified 2 subtypes of thr (thrα and thrβ) with 2 isoforms each (thrαA, thrαB, thrβA, thrβB) and 3 subtypes of deiodinases (dio1, dio2, dio3). All thr genes identified showed high similarity to the closely related Japanese eel (Anguilla japonica). We found that all genes investigated in this study were affected by larval age (in real time or at specific developmental stages), temperature, and/or their interaction. More specifically, the warmer the temperature the earlier the expression response of a specific target gene. In real time, the expression profiles appeared very similar and only shifted with temperature. In developmental time, gene expression of all genes differed across selected developmental stages, such as at hatch, during teeth formation or at first-feeding. Thus, we demonstrate that the expression of thrs and dios show sensitivity to temperature and are involved in and during early life development of European eel.

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Politis Sebastian Nikitas, Servili Arianna, Mazurais David, Zambonino-Infante Jose-Luis, Miest J. J., Tomkiewicz J., Butts I. A. E. (2018). Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel ( Anguilla anguilla ) larvae. General And Comparative Endocrinology, 259, 54-65. Publisher's official version : https://doi.org/10.1016/j.ygcen.2017.11.003 , Open Access version : https://archimer.ifremer.fr/doc/00409/52020/